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Data sheet of Mouse Monoclonal to beta-tubulin / TUBB
| Brand | Exbio |
| Product type | Primary antibodies |
| Reactivity | Human, Porcine, Mouse, Plants |
| Clonality | Monoclonal |
More info about Mouse Monoclonal to beta-tubulin / TUBB
| Brand: | Exbio |
| Product no.: | 11-444-C025 |
| Product type: | Primary antibodies |
| Host species: | Mouse |
| Product name: | Mouse Monoclonal to beta-tubulin / TUBB |
| Antigen: | beta-tubulin |
| Clonality: | Monoclonal |
| Clone: | TU-13 |
| Isotype: | IgM |
| Immunogen: | beta-tubulin from porcine brain |
| Format: | purified |
| Specificity: | The antibody TU-13 recognizes an epitope on N-terminal structural domain of beta-tubulin in various species._x000D_ |
| Categories: | Cytoskeleton: Tubulin System (Rodent), Cytoskeleton: Tubulin System (Human), Cytoskeleton: Microtubular System |
| Concentration: | 1 mg/ml |
| Storage buffer: | Tris buffered saline (TBS) with 15 mM sodium azide, approx. pH 8.0 |
| Storage / stability: | Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label. |
| Background: | The microtubules are intracellular dynamic polymers made up of evolutionarily conserved polymorphic alpha/beta-tubulin heterodimers and a large number of microtubule-associated proteins (MAPs). The microtubules consist of 13 protofilaments and have an outer diameter 25 nm. Microtubules have their intrinsic polarity; highly dynamic plus ends and less dynamic minus ends. Microtubules are required for vital processes in eukaryotic cells including mitosis, meiosis, maintenance of cell shape and intracellular transport. Microtubules are also necessary for movement of cells by means of flagella and cilia. In mammalian tissue culture cells microtubules have their minus ends anchored in microtubule organizing centers (MTOCs).The GTP (guanosintriphosphate) molecule is an essential for tubulin heterodimer to associate with other heterodimers to form microtubule. In vivo, microtubule dynamics vary considerably. Microtubule polymerization is reversible and a populations of microtubules in cells are on their minus ends either growing or shortening – this phenomenon is called dynamic instability of microtubules. On a practical level, microtubules can easily be stabilized by the addition of non-hydrolysable analogues of GTP (eg. GMPPCP) or more commonly by anti-cancer drugs such as Taxol. Taxol stabilizes microtubules at room temperature for many hours. Using limited proteolysis by enzymes both tubulin subunits can be divided into N-terminal and C-terminal structural domains.The beta-tubulin (relative molecular weight around 50 kDa) is counterpart of alpha-tubulin in tubulin heterodimer, it is coded by multiple tubulin genes and it is also posttranslationally modified. Heterogeneity of subunit is concentrated in C-terminal structural domain._x000D_ _x000D_ _x000D_ |
| Purity: | > 95% (by SDS-PAGE) |
| Purification: | Purified by precipitation and chromatography |
| Product specific references: | *Draber P, Lagunowich LA, Draberova E, Viklicky V, Damjanov I: Heterogeneity of tubulin epitopes in mouse fetal tissues. Histochemistry. 1988;89(5):485-92. _x000D_ , *Draber P, Vater W, Bohm KJ, Kuklova E, Unger E: Inhibition of microtubule assembly in vitro by anti-tubulin monoclonal antibodies. FEBS Lett. 1990 Mar 26;262(2):209-11. _x000D_ |
| General references: | *Linhartova I, Draberova E, Viklicky V, Draber P: Distribution of non-class-III beta-tubulin isoforms in neuronal and non-neuronal cells. FEBS Lett. 1993 Mar 29;320(1):79-82. _x000D_ , *Lewis, S.A., Cowan, N.J.: Tubulin genes: structure, expression, and regulation. In: Avila, J. (ed.): Microtubule proteins. Boca Raton: CRC Press, Inc. 1990. Pp. 37-66. _x000D_ _x000D_ |
| Related products: | - Mouse Monoclonal to Blood Group ABH - Mouse Monoclonal to Blood Group A - Mouse Monoclonal to Blood Group A1B |
| Shipping condition: | Room temperature |