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Data sheet of Mouse Monoclonal to beta-tubulin
| Brand | Exbio |
| Product type | Primary antibodies |
| Reactivity | Broad species reactivity |
| Clonality | Monoclonal |
More info about Mouse Monoclonal to beta-tubulin
| Brand: | Exbio |
| Product no.: | 11-251-C025 |
| Product type: | Primary antibodies |
| Host species: | Mouse |
| Product name: | Mouse Monoclonal to beta-tubulin |
| Antigen: | beta-tubulin |
| Clonality: | Monoclonal |
| Clone: | TU-06 |
| Isotype: | IgM |
| Immunogen: | Beta-subunits of porcine brain tubulin. |
| Format: | purified |
| Specificity: | The antibody TU-06 recognizes an epitope (aa 81-95) on phylogenetically conserved N-terminal structural domain of beta-tubulin (recognizes all beta-tubulin isoforms) in various species. |
| Categories: | Cytoskeleton: Tubulin System (Veterinary), Cytoskeleton: Tubulin System (Rodent), Cytoskeleton: Tubulin System (Human), Cytoskeleton: Microtubular System |
| Concentration: | 1 mg/ml |
| Storage buffer: | Tris buffered saline (TBS) with 15 mM sodium azide, approx. pH 8.0 |
| Storage / stability: | Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label. |
| Background: | The microtubules are intracellular dynamic polymers made up of evolutionarily conserved polymorphic alpha/beta-tubulin heterodimers and a large number of microtubule-associated proteins (MAPs). The microtubules consist of 13 protofilaments and have an outer diameter 25 nm. Microtubules have their intrinsic polarity; highly dynamic plus ends and less dynamic minus ends. Microtubules are required for vital processes in eukaryotic cells including mitosis, meiosis, maintenance of cell shape and intracellular transport. Microtubules are also necessary for movement of cells by means of flagella and cilia. In mammalian tissue culture cells microtubules have their minus ends anchored in microtubule organizing centers (MTOCs).The GTP (guanosintriphosphate) molecule is an essential for tubulin heterodimer to associate with other heterodimers to form microtubule. In vivo, microtubule dynamics vary considerably. Microtubule polymerization is reversible and a populations of microtubules in cells are on their minus ends either growing or shortening – this phenomenon is called dynamic instability of microtubules. On a practical level, microtubules can easily be stabilized by the addition of non-hydrolysable analogues of GTP (eg. GMPPCP) or more commonly by anti-cancer drugs such as Taxol. Taxol stabilizes microtubules at room temperature for many hours. Using limited proteolysis by enzymes both tubulin subunits can be divided into N-terminal and C-terminal structural domains._x000D_ The beta-tubulin (relative molecular weight around 50 kDa) is counterpart of alpha-tubulin in tubulin heterodimer, it is coded by multiple tubulin genes and it is also posttranslationally modified. Heterogeneity of subunit is concentrated in C-terminal structural domain._x000D_ _x000D_ _x000D_ |
| Purity: | > 95% (by SDS-PAGE) |
| Purification: | Purified by precipitation and chromatography |
| Product specific references: | *Draber P, Draberova E, Linhartova I, Viklicky V: Differences in the exposure of C- and N-terminal tubulin domains in cytoplasmic microtubules detected with domain-specific monoclonal antibodies. J Cell Sci. 1989 Mar;92 ( Pt 3):519-28. , *Tobita K, Liu LJ, Janczewski AM, Tinney JP, Nonemaker JM, Augustine S, Stolz DB, Shroff SG, Keller BB: Engineered early embryonic cardiac tissue retains proliferative and contractile properties of developing embryonic myocardium. Am J Physiol Heart Circ Physiol. 2006 Oct;291(4):H1829-37., *Libusova L, Sulimenko T, Sulimenko V, Janisch R, Hozak P, Draber P: Distinct localization of a beta-tubulin epitope in the Tetrahymena thermophila and Paramecium caudatum cortex. Protoplasma. 2005 Oct;225(3-4):157-67. _x000D_ , *Smertenko A, Blume Y, Viklicky V, Opatrny Z, Draber P: Post-translational modifications and multiple tubulin isoforms in Nicotiana tabacum L. cells. Planta. 1997;201(3):349-58. _x000D_ , *Draber P, Draberova E, Viklicky V: Immunostaining of human spermatozoa with tubulin domain-specific monoclonal antibodies. Recognition of a unique beta-tubulin epitope in the sperm head. Histochemistry. 1991;95(5):519-24. _x000D_ , *Dryková D, Cenklová V, Sulimenko V, Volc J, Dráber P, Binarová P: Plant gamma-tubulin interacts with alphabeta-tubulin dimers and forms membrane-associated complexes. Plant Cell. 2003 Feb;15(2):465-80., *Smertenko A, Blume Y, Viklický V, Dráber P: Exposure of tubulin structural domains in Nicotiana tabacum microtubules probed by monoclonal antibodies. Eur J Cell Biol. 1997 Feb;72(2):104-12., *Solecki DJ, Model L, Gaetz J, Kapoor TM, Hatten ME: Par6alpha signaling controls glial-guided neuronal migration._x000D_ Nat Neurosci. 2004 Nov;7(11):1195-203. |
| Related products: | - Mouse Monoclonal to Blood Group A1B - Mouse Monoclonal to beta-tubulin / TUBB - Mouse Monoclonal to Blood Group A |
| Shipping condition: | Room temperature |