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Data sheet of Mouse Monoclonal to ARHGEF4 / ASEF1
| Brand | Exbio |
| Product type | Primary antibodies |
| Reactivity | Human |
| Clonality | Monoclonal |
More info about Mouse Monoclonal to ARHGEF4 / ASEF1
| Brand: | Exbio |
| Product no.: | 11-723-C025 |
| Product type: | Primary antibodies |
| Host species: | Mouse |
| Product name: | Mouse Monoclonal to ARHGEF4 / ASEF1 |
| Antigen: | ARHGEF4 |
| Clonality: | Monoclonal |
| Clone: | ARHGEF-08 |
| Isotype: | IgG1 |
| Immunogen: | Recombinant fragment of human ARHGEF4 (amino acids 143-271) |
| Format: | purified |
| Specificity: | The mouse monoclonal antibody ARHGEF-08 recognizes human intracellular protein ARHGEF4 / ASEF1, approx. 80 kDa guanine nucleotide exchange factor specific for Rac1 and Cdc42. |
| Categories: | Signaling of Small GTPases (Human) |
| Concentration: | 1 mg/ml |
| Storage buffer: | Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4 |
| Storage / stability: | Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label. |
| Background: | ARHGEF4 (Rho guanine nucleotide exchange factor 4), also known as ASEF 1 (adenomatous polyposis coli – stimulated guanine nucleotide exchange factor 1) is an approximately 80 kDa cytoplasmic protein important for growth factor-mediated regulation of cell morphology and migration. Besides N-terminal adenomatous polyposis coli (APC)-binding region (ABR) it contains Dbl homology (DH), Pleckstrin homology (PH) and SH3 domains. The SH3 domain inhibits GEF activity of ARHGEF4 by intramolecular interaction with the DH domain, whereas binding of APC stimulates the GEF activity. Activated ARHGEF4 stimulates the small GTPase Cdc42, which leads to decreased cell-cell adherence and enhanced cell migration. |
| Purity: | > 95% (by SDS-PAGE) |
| Purification: | Purified by protein-A affinity chromatography |
| General references: | *Zhang Z, Chen L, Gao L, Lin K, Zhu L, Lu Y, Shi X, Gao Y, Zhou J, Xu P, Zhang J, Wu G: Structural basis for the recognition of Asef by adenomatous polyposis coli. Cell Res. 2012 Feb;22(2):372-86., *Lyons R, Williams O, Morrow M, Sebire N, Hubank M, Anderson J: The RAC specific guanine nucleotide exchange factor Asef functions downstream from TEL-AML1 to promote leukaemic transformation. Leuk Res. 2010 Jan;34(1):109-15., *Kawasaki Y, Tsuji S, Sagara M, Echizen K, Shibata Y, Akiyama T: Adenomatous polyposis coli and Asef function downstream of hepatocyte growth factor and phosphatidylinositol 3-kinase. J Biol Chem. 2009 Aug 14;284(33):22436-43. |
| Related products: | - Mouse Monoclonal to Benzo[a]pyrene - Mouse Monoclonal to Basic Cytokeratins - Mouse Monoclonal to Bcl2 |
| Shipping condition: | Room temperature |