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Data sheet of Mouse Monoclonal to alpha-tubulin
| Brand | Exbio |
| Product type | Primary antibodies |
| Reactivity | Broad species reactivity |
| Clonality | Monoclonal |
More info about Mouse Monoclonal to alpha-tubulin
| Brand: | Exbio |
| Product no.: | 11-283-C025 |
| Product type: | Primary antibodies |
| Host species: | Mouse |
| Product name: | Mouse Monoclonal to alpha-tubulin |
| Antigen: | alpha-tubulin |
| Clonality: | Monoclonal |
| Clone: | TU-16 |
| Isotype: | IgM |
| Immunogen: | Porcine brain microtubule protein MTP-1. |
| Format: | purified |
| Specificity: | The antibody TU-16 reacts with alpha-tubulin of all tested species, under denaturing and non-denaturing conditions. |
| Categories: | Cytoskeleton: Tubulin System (Veterinary), Cytoskeleton: Tubulin System (Rodent), Cytoskeleton: Tubulin System (Human), Cytoskeleton: Microtubular System |
| Concentration: | 1 mg/ml |
| Storage buffer: | Tris buffered saline (TBS) with 15 mM sodium azide, approx. pH 8.0 |
| Storage / stability: | Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label. |
| Background: | The microtubules are intracellular dynamic polymers made up of evolutionarily conserved polymorphic alpha/beta-tubulin heterodimers and a large number of microtubule-associated proteins (MAPs). The microtubules consist of 13 protofilaments and have an outer diameter 25 nm. Microtubules have their intrinsic polarity; highly dynamic plus ends and less dynamic minus ends. Microtubules are required for vital processes in eukaryotic cells including mitosis, meiosis, maintenance of cell shape and intracellular transport. Microtubules are also necessary for movement of cells by means of flagella and cilia. In mammalian tissue culture cells microtubules have their minus ends anchored in microtubule organizing centers (MTOCs).The GTP (guanosintriphosphate) molecule is an essential for tubulin heterodimer to associate with other heterodimers to form microtubule. In vivo, microtubule dynamics vary considerably. Microtubule polymerization is reversible and a populations of microtubules in cells are on their minus ends either growing or shortening – this phenomenon is called dynamic instability of microtubules. On a practical level, microtubules can easily be stabilized by the addition of non-hydrolysable analogues of GTP (eg. GMPPCP) or more commonly by anti-cancer drugs such as Taxol. Taxol stabilizes microtubules at room temperature for many hours. Using limited proteolysis by enzymes both tubulin subunits can be divided into N-terminal and C-terminal structural domains.The alpha-tubulin (relative molecular weight around 50 kDa) is globular protein that exists in cells as part of soluble alpha/beta-tubulin dimer or it is polymerized into microtubules. In different species it is coded by multiple tubulin genes that form tubulin classes (in human 6 genes). Expressed tubulin genes are named tubulin isotypes. Some of the tubulin isotypes are expressed ubiquitously, while some have more restricted tissue expression. Alpha-tubulin is also subject of numerous post-translational modifications. Tubulin isotypes and their posttranslational modifications are responsible for multiple tubulin charge variants - tubulin isoforms. Heterogeneity of alpha-tubulin is concentrated in C-terminal structural domain._x000D_ |
| Purity: | > 95% (by SDS-PAGE) |
| Purification: | Purified by precipitation and chromatography |
| Product specific references: | *Draberova E, Draber P: Novel monoclonal antibodies TU-08 and TU-16 specific for tubulin subunits. Folia Biol (Praha). 1998;44(1):35-6., *Ji Y, Rath U, Girton J, Johansen KM, Johansen J: D-Hillarin, a novel W180-domain protein, affects cytokinesis through interaction with the septin family member Pnut. J Neurobiol. 2005 Aug;64(2):157-69._x000D_ , *Dryková D, Cenklová V, Sulimenko V, Volc J, Dráber P, Binarová P: Plant gamma-tubulin interacts with alphabeta-tubulin dimers and forms membrane-associated complexes. Plant Cell. 2003 Feb;15(2):465-80., *Qi H, Rath U, Wang D, Xu YZ, Ding Y, Zhang W, Blacketer MJ, Paddy MR, Girton J, Johansen J, Johansen KM: Megator, an essential coiled-coil protein that localizes to the putative spindle matrix during mitosis in Drosophila. Mol Biol Cell. 2004 Nov;15(11):4854-65. |
| Related products: | - Mouse Monoclonal to Basic Cytokeratins - Mouse Monoclonal to ARAP1 / centaurin - Mouse Monoclonal to ARHGEF4 / ASEF1 |
| Shipping condition: | Room temperature |