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Data sheet of Mouse Monoclonal to 5-bromodeoxyuridine (BrdU)
| Brand | Exbio |
| Product type | Primary antibodies |
| Clonality | Monoclonal |
More info about Mouse Monoclonal to 5-bromodeoxyuridine (BrdU)
| Brand: | Exbio |
| Product no.: | 11-682-C025 |
| Product type: | Primary antibodies |
| Host species: | Mouse |
| Product name: | Mouse Monoclonal to 5-bromodeoxyuridine (BrdU) |
| Antigen: | 5-bromodeoxyuridine (BrdU) |
| Clonality: | Monoclonal |
| Clone: | Bu20a |
| Isotype: | IgG1 |
| Immunogen: | Bromodeoxyuridine conjugated to BSA |
| Format: | purified |
| Specificity: | The antibody Bu20a reacts specifically with BrdU incorporated into DNA during S-phase of a cell cycle. It is useful for detecting proliferating cells by flow cytometry or immunohistochemistry staining. |
| Categories: | Small Organic Molecules |
| Concentration: | 1 mg/ml |
| Storage buffer: | Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4 |
| Storage / stability: | Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label. |
| Background: | Bromodexyuridine (BrdU) is a thymidine analog which is selectively incorporated into the DNA of proliferating cells to provide a marker for the DNA being replicated. The number of proliferating cells can then be detected in cell lysates, tissue sections or suspensions using an antibody specific for the BrdU. |
| Purity: | > 95% (by SDS-PAGE) |
| Purification: | Purified by protein-A affinity chromatography |
| Product specific references: | *And many other., *Magaud JP, Sargent I, Clarke PJ, Ffrench M, Rimokh R, Mason DY: Double immunocytochemical labeling of cell and tissue samples with monoclonal anti-bromodeoxyuridine. J Histochem Cytochem. 1989 Oct;37(10):1517-27., *Yatera K, Hsieh J, Hogg JC, Tranfield E, Suzuki H, Shih CH, Behzad AR, Vincent R, van Eeden SF: Particulate matter air pollution exposure promotes recruitment of monocytes into _x000D_ atherosclerotic plaques. Am J Physiol Heart Circ Physiol. 2008 Feb;294(2):H944-53., *MartÃn CA, Surur JM, GarcÃa MN, Corrons F, Badrán AF: Comparative study of DNA synthesis and nucleolar organizer regions of sinusoid littoral cells in mouse regenerating liver. Biocell. 2005 Apr;29(1):33-8., *Shih CH, Whalen BA, Goto Y, Hogg JC, van Eeden SF: Flow cytometric method for enumeration and characterization of newly released polymorphonuclear leukocytes from the bone marrow using 5'-bromo-2'-deoxyuridine. Am J Physiol Cell Physiol. 2005 Sep;289(3):C757-65., *Lagares A, Li HY, Zhou XF, Avendaño C: Primary sensory neuron addition in the adult rat trigeminal ganglion: evidence_x000D_ for neural crest glio-neuronal precursor maturation. J Neurosci. 2007 Jul 25;27(30):7939-53., *Muskhelishvili L, Latendresse JR, Kodell RL, Henderson EB: Evaluation of cell proliferation in rat tissues with BrdU, PCNA, Ki-67(MIB-5) immunohistochemistry and in situ hybridization for histone mRNA. J Histochem Cytochem. 2003 Dec;51(12):1681-8., *Pajalunga D, Mazzola A, Salzano AM, Biferi MG, De Luca G, Crescenzi M: Critical requirement for cell cycle inhibitors in sustaining nonproliferative states. J Cell Biol. 2007 Mar 12;176(6):807-18., O'Driscoll M, Jeggo PA: CsA can induce DNA double-strand breaks: implications for BMT regimens_x000D_ particularly for individuals with defective DNA repair. Bone Marrow Transplant. 2008 Jun;41(11):983-9. |
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| Shipping condition: | Room temperature |