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Data sheet of Mouse Monoclonal to 5-bromodeoxyuridine (BrdU)
| Brand | Exbio |
| Product type | Primary antibodies |
| Clonality | Monoclonal |
More info about Mouse Monoclonal to 5-bromodeoxyuridine (BrdU)
| Brand: | Exbio |
| Product no.: | 11-286-C025 |
| Product type: | Primary antibodies |
| Host species: | Mouse |
| Product name: | Mouse Monoclonal to 5-bromodeoxyuridine (BrdU) |
| Antigen: | 5-bromodeoxyuridine (BrdU) |
| Clonality: | Monoclonal |
| Clone: | MoBu-1 |
| Isotype: | IgG1 |
| Immunogen: | 5-bromodeoxyuridine conjugated with hemocyanine. |
| Format: | purified |
| Specificity: | The antibody MoBu-1 reacts specifically with BrdU incorporated into DNA during S-phase of a cell cycle. The antibody MoBu-1 is also useful for detecting proliferating cells by flow cytometry or immunofluorescence staining. It reacts also specifically with 5-bromouridine (BrU). |
| Categories: | Transcription Regulation & Nuclear Proteins, Small Organic Molecules |
| Concentration: | 1 mg/ml |
| Storage buffer: | Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4 |
| Storage / stability: | Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label. |
| Background: | Bromodexyuridine (BrdU) is a thymidine analog which is selectively incorporated into the DNA of proliferating cells to provide a marker for the DNA being replicated. The number of proliferating cells can then be detected in cell lysates, tissue sections or suspensions using an antibody specific for the BrdU. |
| Purity: | > 95% (by SDS-PAGE) |
| Purification: | Purified by protein-A affinity chromatography |
| Product specific references: | *Stanek D, Kiss T, Raska I: Pre-ribosomal RNA is processed in permeabilised cells at the site of transcription. Eur J Cell Biol. 2000 Mar;79(3):202-7. , *Buckiova D, Kubinova L, Soukup A, Jelinek R, Brown NA: Hyperthermia in the chick embryo: HSP and possible mechanisms of developmental defects. Int J Dev Biol. 1998 Jul;42(5):737-40. |
| General references: | *Soames AR, Lavender D, Foster JR, Williams SM, Wheeldon EB: Image analysis of bromodeoxyuridine (BrdU) staining for measurement of S-phase in rat and mouse liver. J Histochem Cytochem. 1994 Jul;42(7):939-44. _x000D_ , *Ashby J, Tinwell H, Soames A, Foster J: Induction of hyperplasia and increased DNA content in the uterus of immature rats exposed to coumestrol. Environ Health Perspect. 1999 Oct;107(10):819-22. _x000D_ |
| Related products: | - Mouse Monoclonal to Acrosin (Porcine) - Mouse Monoclonal to ABRA1 - Mouse Monoclonal to Acidic Cytokeratins |
| Shipping condition: | Room temperature |