CD45RA monoclonal antibody, clone SPM504 View larger

CD45RA monoclonal antibody, clone SPM504

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289,00 € tax excl.

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Data sheet of CD45RA monoclonal antibody, clone SPM504

BrandAbnova
Product typePrimary antibodies
ReactivityHuman
ClonalityMonoclonal
Host speciesMouse
ApplicationsIHC-P,IF,Flow Cyt

More info about CD45RA monoclonal antibody, clone SPM504

Product description: Mouse monoclonal antibody raised against native human CD45RA.
Clone: SPM504
Isotype: IgG1, kappa
Gene id: 5788
Gene name: PTPRC
Gene alias: B220|CD45|CD45R|GP180|LCA|LY5|T200
Gene description: protein tyrosine phosphatase, receptor type, C
Immunogen: Stimulated human leukocytes.
Form: Liquid
Recommend dilutions: Flow Cytometry (0.5-1 ug/106 cells in 0.1 mL)
Immunofluorescence (1-2 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (0.5-1 ug/mL)
The optimal working dilution should be determined by the end user.
Storage buffer: In 10 mM PBS (0.05% BSA, 0.05% sodium azide).
Storage instruction: Store at 4°C.
Note: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Specificity: Recognizes a protein of 205 kDa-220 kDa, identified as CD45RA (Workshop III). CD45RA is isoforms of the human leukocyte common antigen (CD45). Human CD45 contains three exons which encode peptide segments designated A, B and C, respectively. The differential splicing of the exons generates at least five isoforms, ABC, AB, BC, B and O. This antibody reacts with ABC and BC isoforms. CD45RA is expressed on 40-50% of peripheral CD4+ T cells, 50% of peripheral CD8+ T cells, B cells, and leukemic B cell lines. T cells expressing CD45RA are naive or virgin T cells. T cells expressing CD45RO are memory T cells. CD45RA and CD45RO define complementary, predominantly non-overlapping populations of resting peripheral T cells. This monoclonal antibody is useful in study on the subpopulation of CD4+ or CD8+ T cells. It can especially be used to differentiate T cell lymphomas (CD45RO+ve) from B cell lymphomas (CD45RA+ve).
Size: 100 ug
Shipping condition: Blue Ice

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