Brand | Abnova |
Product type | Primary antibodies |
Reactivity | Human |
Clonality | Monoclonal |
Host species | Mouse |
Applications | IHC-P,IF,Flow Cyt |
Product description: | Mouse monoclonal antibody raised against native human CD45RA. |
Clone: | SPM504 |
Isotype: | IgG1, kappa |
Gene id: | 5788 |
Gene name: | PTPRC |
Gene alias: | B220|CD45|CD45R|GP180|LCA|LY5|T200 |
Gene description: | protein tyrosine phosphatase, receptor type, C |
Immunogen: | Stimulated human leukocytes. |
Form: | Liquid |
Recommend dilutions: | Flow Cytometry (0.5-1 ug/106 cells in 0.1 mL) Immunofluorescence (1-2 ug/mL) Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (0.5-1 ug/mL) The optimal working dilution should be determined by the end user. |
Storage buffer: | In 10 mM PBS (0.05% BSA, 0.05% sodium azide). |
Storage instruction: | Store at 4°C. |
Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
Specificity: | Recognizes a protein of 205 kDa-220 kDa, identified as CD45RA (Workshop III). CD45RA is isoforms of the human leukocyte common antigen (CD45). Human CD45 contains three exons which encode peptide segments designated A, B and C, respectively. The differential splicing of the exons generates at least five isoforms, ABC, AB, BC, B and O. This antibody reacts with ABC and BC isoforms. CD45RA is expressed on 40-50% of peripheral CD4+ T cells, 50% of peripheral CD8+ T cells, B cells, and leukemic B cell lines. T cells expressing CD45RA are naive or virgin T cells. T cells expressing CD45RO are memory T cells. CD45RA and CD45RO define complementary, predominantly non-overlapping populations of resting peripheral T cells. This monoclonal antibody is useful in study on the subpopulation of CD4+ or CD8+ T cells. It can especially be used to differentiate T cell lymphomas (CD45RO+ve) from B cell lymphomas (CD45RA+ve). |
Size: | 100 ug |
Shipping condition: | Blue Ice |