Brand | Abnova |
Product type | Primary antibodies |
Reactivity | Human |
Clonality | Monoclonal |
Host species | Mouse |
Applications | WB,IHC-P,IF,Flow Cyt |
Product description: | Mouse monoclonal antibody raised against native human HLA-DRB1. |
Clone: | SPM288 |
Isotype: | IgG2b, kappa |
Gene id: | 3123 |
Gene name: | HLA-DRB1 |
Gene alias: | DRB1|FLJ76359|HLA-DR1B|HLA-DRB|HLA-DRB1*|SS1 |
Gene description: | major histocompatibility complex, class II, DR beta 1 |
Immunogen: | Activated human peripheral blood mononuclear cells. |
Protein accession: | P01911 |
Form: | Liquid |
Recommend dilutions: | Flow Cytometry (0.5-1 ug/106 cells in 0.1 mL) Immunofluorescence (0.5-1 ug/mL) Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (0.25-0.5 ug/mL) Western Blot (0.5-1 ug/mL) The optimal working dilution should be d |
Storage buffer: | In 10 mM PBS. |
Storage instruction: | Store at -20 to -80°C. Aliquot to avoid repeated freezing and thawing. |
Specificity: | This monoclonal antibody does not cross react with HLA-DP and HLA-DQ. HLA-DR is a heterodimeric cell surface glycoprotein comprised of a 36 kDa alpha (heavy) chain and a 28 kDa beta (light) chain. It is expressed on B cells, activated T cells, monocytes/macrophages, dendritic cells and other non-professional APCs. In conjunction with the CD3/TCR complex and CD4 molecules, HLA-DR is critical for efficient peptide presentation to CD4+ T cells. It is an excellent histiocytic marker in paraffin sections producing intense staining. True histiocytic neoplasms are similarly positive. HLA-DR antigens also occur on a variety of epithelial cells and their corresponding neoplastic counterparts. Loss of HLA-DR expression is related to tumor microenvironment and predicts adverse outcome in diffuse large B cell lymphoma. |
Size: | 100 ug |
Shipping condition: | Dry Ice |