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Data sheet of ECM1 monoclonal antibody, clone SC05
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Human,Rat |
| Host species | Mouse |
| Applications | WB,IP,ELISA,ICC,IHC-P,IF |
More info about ECM1 monoclonal antibody, clone SC05
| Brand: | Abnova |
| Reference: | MAB11329 |
| Product name: | ECM1 monoclonal antibody, clone SC05 |
| Product description: | Mouse monoclonal antibody raised against ECM1. |
| Clone: | SC05 |
| Isotype: | IgG1, kappa |
| Gene id: | 1893 |
| Gene name: | ECM1 |
| Gene alias: | - |
| Gene description: | extracellular matrix protein 1 |
| Immunogen: | Partially purified ECM1 from human colostrum (Bartek, 1989). |
| Form: | Liquid |
| Recommend dilutions: | Western Blot (1:100-1:2000) Electron Microscopy (1:10-1:500) ELISA (1:100-1:2000) Immunocytochemistry/Immunofluorescence (1:10-1:2000) Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:500) Immunoprecipitation (1:10-1: |
| Storage buffer: | In PBS (0.05% BSA, 0.05% sodium azide) |
| Storage instruction: | Store at 4°C. Aliquot to avoid repeated freezing and thawing. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Mouse |
| Antigen species / target species: | Human |
| Specificity: | The antibody reacts with a reduction-resistant epitope present in both free and SIgA bound Secretory Component. However, the exact epitope recognized by the antibody has not been mapped. |
| Reactivity: | Human,Rat |
| Application image: |  |
| Application image note: | Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) analysis of human small intestine with ECM1 monoclonal antibody, clone SC05 (Cat # MAB11329) at 1:500 using peroxidase-conjugate and DAB chromogen (Note: cytoplasmic and membrane staining). Immunohistochemical staining of formalin/paraffin tissues is enhanced by unmasking of the target epitope with trypsin at 1 mg/ml for 5 min at 37°C. Note: enzyme digestion may be better than citrate buffer based epitope unmasking according to Brown, 1993. However, we have found antigen retrieval with Heat Induced Epitope Retrieval (HIER) in Citrate buffer (pH6.0) also works well. In this case, de-paraffinized tissue sections should be boiled in this buffer for 5 minutes followed by cooling at room temp for 20 minutes. |
| Applications: | WB,IP,ELISA,ICC,IHC-P,IF |
| Shipping condition: | Blue Ice |