ECM1 monoclonal antibody, clone SC05 View larger

ECM1 monoclonal antibody, clone SC05

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Data sheet of ECM1 monoclonal antibody, clone SC05

BrandAbnova
Product typePrimary antibodies
ReactivityHuman,Rat
Host speciesMouse
ApplicationsWB,IP,ELISA,ICC,IHC-P,IF

More info about ECM1 monoclonal antibody, clone SC05

Brand: Abnova
Reference: MAB11329
Product name: ECM1 monoclonal antibody, clone SC05
Product description: Mouse monoclonal antibody raised against ECM1.
Clone: SC05
Isotype: IgG1, kappa
Gene id: 1893
Gene name: ECM1
Gene alias: -
Gene description: extracellular matrix protein 1
Immunogen: Partially purified ECM1 from human colostrum (Bartek, 1989).
Form: Liquid
Recommend dilutions: Western Blot (1:100-1:2000)
Electron Microscopy (1:10-1:500)
ELISA (1:100-1:2000)
Immunocytochemistry/Immunofluorescence (1:10-1:2000)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:500)
Immunoprecipitation (1:10-1:
Storage buffer: In PBS (0.05% BSA, 0.05% sodium azide)
Storage instruction: Store at 4°C.
Aliquot to avoid repeated freezing and thawing.
Note: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Product type: Primary antibodies
Host species: Mouse
Antigen species / target species: Human
Specificity: The antibody reacts with a reduction-resistant epitope present in both free and SIgA bound Secretory Component. However, the exact epitope recognized by the antibody has not been mapped.
Reactivity: Human,Rat
Application image: MAB11329-48-36-1.jpg
Application image note: Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) analysis of human small intestine with ECM1 monoclonal antibody, clone SC05 (Cat # MAB11329) at 1:500 using peroxidase-conjugate and DAB chromogen (Note: cytoplasmic and membrane staining). Immunohistochemical staining of formalin/paraffin tissues is enhanced by unmasking of the target epitope with trypsin at 1 mg/ml for 5 min at 37°C. Note: enzyme digestion may be better than citrate buffer based epitope unmasking according to Brown, 1993. However, we have found antigen retrieval with Heat Induced Epitope Retrieval (HIER) in Citrate buffer (pH6.0) also works well. In this case, de-paraffinized tissue sections should be boiled in this buffer for 5 minutes followed by cooling at room temp for 20 minutes.
Applications: WB,IP,ELISA,ICC,IHC-P,IF
Shipping condition: Blue Ice

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