Brand: | Abnova |
Reference: | MAB0960 |
Product name: | LIME1 monoclonal antibody, clone LIME-06 |
Product description: | Mouse monoclonal antibody raised against partial recombinant LIME1. |
Clone: | LIME-06 |
Isotype: | IgG1 |
Gene id: | 54923 |
Gene name: | LIME1 |
Gene alias: | FLJ20406|LIME|LP8067|dJ583P15.4 |
Gene description: | Lck interacting transmembrane adaptor 1 |
Immunogen: | Recombinant protein corresponding to amino acids 141-295 of human LIME1. |
Form: | Liquid |
Recommend dilutions: | The optimal working dilution should be determined by the end user. |
Storage buffer: | In PBS, pH 7.4 (0.09% sodium azide) |
Storage instruction: | Store at 4°C. Do not freeze. Aliquot to avoid repeated freezing and thawing. |
Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
Product type: | Primary antibodies |
Host species: | Mouse |
Antigen species / target species: | Human |
Specificity: | This antibody was raised against intracellular fragment corresponding to aa 141-295 of human LIME, a 31 KDa Lck-interacting transmembrane adaptor expressed by T cells. |
Reactivity: | Human |
Application image: |  |
Application image note: | Induction of LIME1 tyrosine phosphorylation. Peripheral blood T cells were left unstimulated (-) or stimulated with anti-human CD3 (MEM-92) or anti-human CD4 (MEM-16), and LIME1 was immunoprecipitated from laurylmaltoside lysates with the LIME1 monoclonal antibody, clone LIME-06 (Cat # MAB0960) (immunoaffinity sorbent) and analyzed by Western blotting to visualize tyrosine-phosphorylated LIME1 (top) and total LIME1 (bottom). |
Applications: | IP |
Shipping condition: | Blue Ice |