NFKBIA (phospho S32/S36) monoclonal antibody, clone 39A1413 View larger

NFKBIA (phospho S32/S36) monoclonal antibody, clone 39A1413

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Data sheet of NFKBIA (phospho S32/S36) monoclonal antibody, clone 39A1413

BrandAbnova
Product typePrimary antibodies
ReactivityHuman,Mouse
Host speciesMouse
ApplicationsIP,ELISA,WB-Ce

More info about NFKBIA (phospho S32/S36) monoclonal antibody, clone 39A1413

Brand: Abnova
Reference: MAB0056
Product name: NFKBIA (phospho S32/S36) monoclonal antibody, clone 39A1413
Product description: Mouse monoclonal antibody raised against synthetic phosphopeptide of NFKBIA.
Clone: 39A1413
Isotype: IgG1
Gene id: 4792
Gene name: NFKBIA
Gene alias: IKBA|MAD-3|NFKBI
Gene description: nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha
Immunogen: Synthetic phosphopeptide corresponding to residues surrounding S32/S36 of human NFKBIA.
Form: Liquid
Recommend dilutions: The optimal working dilution should be determined by the end user.
Storage buffer: In PBS (0.05% BSA, 0.05% sodium azide)
Storage instruction: Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
Note: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Product type: Primary antibodies
Host species: Mouse
Antigen species / target species: Human
Reactivity: Human,Mouse
Application image: MAB0056-46-6-1.jpg
Application image note: Jurkat cells were treated for 30 min with 100 ug/mL ALLN (N-Acetyl-Leu-Leu-Norleucinal; a Calpain inhibitor and also proteasome inhibitor that prevents IkBa dephosphorylation) followed by incubation with (lanes 2 & 4) or without 1 nM TNF-a (1 & 3). The membranes were blotted with NFKBIA monoclonal antibody, clone 39A1413 (Cat # MAB0056) (lanes 1 & 2) or NFKBIA monoclonal antibody, clone 6A920 (Cat # MAB0057) (that recognizes both non-phospho and phosphorylated forms of IkBa) and immunoreactivity was detected by ECL. The data shows that Cat # MAB0056 detects specifically the phosphorylated form of NFKBIA.
Applications: IP,ELISA,WB-Ce
Shipping condition: Dry Ice
Publications: Cardioprotection afforded by inducible nitric oxide synthase gene therapy is mediated by cyclooxygenase-2 via a nuclear factor-kappaB dependent pathway.Li Q, Guo Y, Tan W, Ou Q, Wu WJ, Sturza D, Dawn B, Hunt G, Cui C, Bolli R.
Circulation. 2007 Oct 2;116(14):1577-84. Epub 2007 Sep 4.

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