Brand | Abnova |
Product type | Primary antibodies |
Reactivity | Human |
Host species | Mouse |
Applications | ELISA,WB-Ce,WB-Re |
Brand: | Abnova |
Reference: | MAB0011 |
Product name: | TP53 (phospho S46) monoclonal antibody, clone TK-4D4 |
Product description: | Mouse monoclonal antibody raised against synthetic phosphopeptide of TP53. |
Clone: | TK-4D4 |
Isotype: | IgG1 |
Gene id: | 7157 |
Gene name: | TP53 |
Gene alias: | FLJ92943|LFS1|TRP53|p53 |
Gene description: | tumor protein p53 |
Genbank accession: | X60014 |
Immunogen: | Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding S46 of human TP53. |
Protein accession: | CAA42629 |
Form: | Liquid |
Recommend dilutions: | Western Blot (1-2 ug/mL) ELISA (1 ug/mL) The optimal working dilution should be determined by the end user. |
Storage buffer: | In 10 mM HEPES/KOH, 150 mM NaCl, pH 7.5 (50% glycerol) |
Storage instruction: | Store at -20°C. Aliquot to avoid repeated freezing and thawing. |
Quality control testing: | Phospho-p53 Ser46 Antibody detects phosphorylated p53 at serine 46 in an immunoprecipitates using anti-p53 specific polyclonal antibody, by western blotting or phosphorylated recombinant p53 only when phosphorylated at serine 46 in vitro, by western blotting. |
Product type: | Primary antibodies |
Host species: | Mouse |
Antigen species / target species: | Human |
Reactivity: | Human |
Application image: | ![]() |
Application image note: | H2O2 and Adriamycin treatment induce phosphorylation of p53 at serine 46 in MCF-7 cells which expresses wild type p53. Detected by Immunoprecipitation-western blotting using anti-p53 polyclonal antibody and p53 (phospho S46) monoclonal antibody, clone TK-4D4 (Cat # MAB0011). |
Applications: | ELISA,WB-Ce,WB-Re |
Shipping condition: | Dry Ice |