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Data sheet of 293T whole cell lysate (denatured)
| Brand | Abnova |
| Product type | Lysate |
| Host species | Human |
| Applications | WB |
More info about 293T whole cell lysate (denatured)
| Brand: | Abnova |
| Reference: | L151V1 |
| Product name: | 293T whole cell lysate (denatured) |
| Product description: | Whole cell lysate (denatured) |
| Lysis buffer: | 1X Sample Buffer (0.2 M Tris-HCl, 2% SDS, 10% glycerol, 0.2% 2-mercaptoethanol, 0.2% Bromophenol blue). |
| Tissue: | Kidney |
| Preparation method: | Culture cells in 6-well plate to 80% confluent, remove media and then rinse with cold 1x PBS. Scrape cells off the plate surface and pipette cells into microcentrifuge tube to spin down the cells. Remove PBS and then lysis and resuspend cells with 205 ul 1X sample buffer. Mix well and heat at 90°C for 10 minutes, and then cooled rapidly. |
| Recommend dilutions: | The lysate is ready to load on SDS-PAGE for Western blotting. |
| Storage buffer: | In ready-to-use 1X Sample Buffer (0.2 M Tris-HCl, 2% SDS, 10% glycerol, 0.2% 2-mercaptoethanol, 0.2% Bromophenol blue). |
| Storage instruction: | Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | 12.5% SDS-PAGE Stained with Coomassie Blue. |
| Quality control testing picture: |  |
| Product type: | Lysate |
| Host species: | Human |
| Antigen species / target species: | Human |
| Applications: | WB |
| Shipping condition: | Dry Ice |
| Publications: | Autologous T cells expressing the oncogenic transcription factor KLF6-SV1 prevent apoptosis of chronic lymphocytic leukemia cells.Kokhaei P, Hojjat-Farsangi M, Mozaffari F, Moshfegh A, Pak F, Rashidy-Pour A, Palma M, Hansson L, Osterborg A, Mellstedt H. PLoS One. 2018 Feb 12;13(2):e0192839. |