MAP3K5 HEK293 Cell Transient Overexpression Lysate(Non-Denatured) View larger

MAP3K5 HEK293 Cell Transient Overexpression Lysate(Non-Denatured)

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Data sheet of MAP3K5 HEK293 Cell Transient Overexpression Lysate(Non-Denatured)

BrandAbnova
Product typeLysate
Origin speciesHuman
Host speciesHuman
ApplicationsWB,IP

More info about MAP3K5 HEK293 Cell Transient Overexpression Lysate(Non-Denatured)

Reference: L141T6
Product name: MAP3K5 HEK293 Cell Transient Overexpression Lysate(Non-Denatured)
Gene id: 4217
Gene name: MAP3K5
Gene alias: ASK1|MAPKKK5|MEKK5
Gene description: mitogen-activated protein kinase kinase kinase 5
Genbank accession: BC054503
Protein accession: AAH54503
Concentration: 2 mg/ml
Plasmid: pCMV-MAP3K5 full length
Lysis buffer: Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Host cell: HEK293
Recommend dilutions: Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Storage buffer: In modified RIPA Lysis Buffer.
Storage instruction: Store at -80°C. Aliquot to avoid repeated freezing and thawing.
Quality control testing: Transient overexpression cell lysate was tested with Anti-MAP3K5 antibody (H00004217-M03) by Western Blots.
Shipping condition: Dry Ice

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