Quality control testing picture note: | Standard curve of Ethanol Assay Kit (Cat # KA4087) was measured with 96-well black plate using a Gemini fluorescence microplate reader (Molecular Devices). As low as 0.0003% of Ethanol was detected with 15 minutes incubation time (n=3). The fluorescence in blank wells (with the H2O only) is used as a control, and is subtracted from the values for those wells with ethanol reactions. Note: The fluorescence background increases with time, thus it is important to subtract the fluorescence intensity value of the blank wells for each data point. |