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Brand | Abnova |
Product type | Elisa & Assay kits |
Applications | Quant |
Brand: | Abnova |
Reference: | KA4075 |
Product name: | Intracellular Total ROS Activity Assay Kit (Orange Fluorescence) |
Product description: | Intracellular Total ROS Activity Assay Kit (Orange Fluorescence) is used for the measurement of intracellular total ROS activity. |
Storage instruction: | Store the kit in desiccated environment at -20°C and avoid from light. |
Quality control testing picture: | ![]() |
Quality control testing picture note: | Top: Hela cells were seeded overnight at 15,000 cells/90uL/well in a Costar black wall/clear bottom 96-well plate. The cells were untreated (control) or treated with1 mM H2O2 or 100 uM tert-butyl hydroperoxide (TBHP) for 30 min at 37°C. The ROS 570 assay solution (100uL/well) was added and incubated in a 5% CO2, 37°C incubator for 1 hour. The fluorescence signal were monitored at Ex/Em = 540/570 nm (cut off = 550 nm) with bottom read mode using FlexStation (Molecular Devices). Bottom: Images of Hela cells stained with the Intracellular Total ROS Activity Assay Kit (Orange Fluorescence) (Cat # KA4075) in a Costar black wall/clear bottom 96-well plate. A: Untreated control cells. B: Cells treated with 100 uM tert-butyl hydroperoxide (TBHP) for 30min before staining. |
Note: | Detection of ROS in Hela cells. |
Product type: | Elisa & Assay kits |
Applications: | Quant |
Shipping condition: | Dry Ice |