Brand: | Abnova |
Reference: | KA3841 |
Product name: | ROS Activity Assay Kit |
Product description: | ROS Activity Assay Kit is a quantitative fluorometric determination of mitochondrial ROS activity. |
Storage instruction: | Store the kit at -20°C. For best results, please store the components as described in the protocol. |
Quality control testing picture: | |
Quality control testing picture note: | Detection of ROS in Hela cells. Hela cells were seeded overnight at 15000 cells/90 uL/well in a Costar black wall/clear bottom 96-well plate. The cells were treated (1 mM H2O2 or 100 uM tert-butyl hydroperoxide) or untreated (control) for 30 min at 37°C. The ROS Deep Red stain solution (100 uL/well) was added and incubated in a 5% CO2, 37°C incubator for 1 hour. The fluorescence signal was monitored at Ex/Em = 650/675 nm (cut off = 665 nm) with bottom read mode using FlexStation. |
Note: | Result Data |
Product type: | Elisa & Assay kits |
Applications: | Func,Flow Cyt |
Shipping condition: | Dry Ice |