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Data sheet of Cholesterol Cell-Based Detection Assay Kit
| Brand | Abnova |
| Product type | Elisa & Assay kits |
| Applications | IF,Func |
More info about Cholesterol Cell-Based Detection Assay Kit
| Brand: | Abnova |
| Reference: | KA1304 |
| Product name: | Cholesterol Cell-Based Detection Assay Kit |
| Product description: | Cholesterol Cell-Based Detection Assay Kit is a fluorescence-labeled method for histochemical identification of unesterified cholesterol in vivo and in vitro. |
| Storage instruction: | Store the kit at -20°C temporarily. For best results, store the components as described in the protocol. |
| Product type: | Elisa & Assay kits |
| Application image: |  |
| Application image note: | Accumulation of cholesterol inside HepG2 cells in response to 1.25 uM U18666A. HepG2 cells were seeded in a 96-well plate at a density of 3 X 104 cells/well and cultured overnight. The next day, cells were treated with DMSO (vehicle) or 1.25 uM U18666A for 48 hours. Panel A : Cells treated with DMSO alone demonstrate that majority of cholesterol is localized on the plasma membrane. Panel B : U18666A treatment for 48 hours induces intracellular accumulation of cholesterol droplets, indicating blockage of intracellular cholesterol transport. |
| Applications: | IF,Func |
| Shipping condition: | Dry Ice |
| Publications: | Glycerophospholipid profile in oncogene-induced senescence.Cadenas C, Vosbeck S, Hein EM, Hellwig B, Langer A, Hayen H, Franckenstein D, Buttner B, Hammad S, Marchan R, Hermes M, Selinski S, Rahnenfuhrer J, Peksel B, Torok Z, Vigh L, Hengstler JG. Biochim Biophys Acta. 2011 Dec 10. |