Brand | Abnova |
Product type | Primary antibodies |
Reactivity | Human,Mouse,Rat |
Host species | Rabbit |
Applications | IP,ELISA,WB-Ce,IHC-P |
Brand: | Abnova |
Reference: | PAB9943 |
Product name: | CCNB1 polyclonal antibody |
Product description: | Rabbit polyclonal antibody raised against full length recombinant CCNB1. |
Gene id: | 891 |
Gene name: | CCNB1 |
Gene alias: | CCNB |
Gene description: | cyclin B1 |
Immunogen: | Recombinant GST fusion protein corresponding to full length human CCNB1. |
Protein accession: | NP_114172;P14635 |
Form: | Liquid |
Recommend dilutions: | ELISA (1:2000-1:10000) Western Blot (1:500-1:1000) The optimal working dilution should be determined by the end user. |
Storage buffer: | In antiserum (0.01% sodium azide) |
Storage instruction: | Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. |
Quality control testing: | Antibody Reactive Against Recombinant Protein. |
Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
Product type: | Primary antibodies |
Host species: | Rabbit |
Antigen species / target species: | Human |
Reactivity: | Human,Mouse,Rat |
Application image: | ![]() |
Application image note: | Western blot analysis using CCNB1 polyclonal antibody (Cat # PAB9943) antibody shows detection of human CCNB1 present in asynchronous HN30 cell lysates. HN30 cells are from head and neck cancer tumors that over express CCNB1 and D1. Comparison to a molecular weight marker indicates a band of ~62 KDa corresponding to the expected molecular weight for the protein (arrowhead). The blot was incubated with a 1 : 500 dilution of the antibody for 1 h at room temperature. Detection occurred using a 1 : 10,000 of HRP conjugated Goat-a-Rabbit IgG and chemiluminescence reagent with a 1-min exposure time. Personal communication, Luca Cote, Temple University, Philadelphia, PA. |
Applications: | IP,ELISA,WB-Ce,IHC-P |
Shipping condition: | Dry Ice |
Publications: | A 60 kd cdc2-associated polypeptide complexes with the E1A proteins in adenovirus-infected cells.Giordano A, Whyte P, Harlow E, Franza BR Jr, Beach D, Draetta G. Cell. 1989 Sep 8;58(5):981-90. |