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Data sheet of CDK2 polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Human,Mouse,Rat |
| Host species | Rabbit |
| Applications | IP,ELISA,WB-Ce,IHC-P |
More info about CDK2 polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB9940 |
| Product name: | CDK2 polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against synthetic peptide of CDK2. |
| Gene id: | 1017 |
| Gene name: | CDK2 |
| Gene alias: | p33(CDK2) |
| Gene description: | cyclin-dependent kinase 2 |
| Immunogen: | A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human CDK2. |
| Form: | Liquid |
| Recommend dilutions: | ELISA (1:1000-1:5000) Western Blot (1:200-1:1000) Immunohistochemistry (1:200-1:1000) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In antiserum (0.01% sodium azide) |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Synthetic Peptide. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Human |
| Specificity: | Antiserum will specifically react with a 33 KDa cdk2 protein from human, rat and mouse tissue. Cross reactivity with cdk2 from other species may also occur. |
| Reactivity: | Human,Mouse,Rat |
| Application image: |  |
| Application image note: | Immunoblotting of CDK2 polyclonal antibody (Cat # PAB9940) was used at a 1 : 200 dilution to detect CDK2 in asynchronous HeLa cell lysates (run in duplicate). Approximately 50 ug of lysate was separated on a 15% SDS-PAGE gel. CDK2 is indicated by an arrowhead as a 32-33 KDa band. Note that multiple isoforms as well as phosphorylated forms of CDK2 may be detected by this antibody. Primary antibody was reacted at room temperature for 1 h. After subsequent washing, a 1 : 5,000 dilution of HRP conjugated Gt-a-Rabbit IgG preceded color development. |
| Applications: | IP,ELISA,WB-Ce,IHC-P |
| Shipping condition: | Dry Ice |
| Publications: | Cell cycle regulation of histone H1 kinase activity associated with the adenoviral protein E1A.Giordano A, Lee JH, Scheppler JA, Herrmann C, Harlow E, Deuschle U, Beach D, Franza BR Jr. Science. 1991 Sep 13;253(5025):1271-5. |