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Data sheet of CDC2 polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Human,Mouse,Rat |
| Host species | Rabbit |
| Applications | IP,ELISA,IHC,WB-Ce |
More info about CDC2 polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB9939 |
| Product name: | CDC2 polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against synthetic peptide of CDC2. |
| Gene id: | 983 |
| Gene name: | CDC2 |
| Gene alias: | CDC28A|CDK1|DKFZp686L20222|MGC111195 |
| Gene description: | cell division cycle 2, G1 to S and G2 to M |
| Immunogen: | A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human CDC2. |
| Form: | Liquid |
| Recommend dilutions: | ELISA (1:500-1:2000) Western Blot (1:500-1:1000) Immunohistochemistry (1:200-1:1000) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In antiserum (0.01% sodium azide) |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Synthetic Peptide. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Human |
| Specificity: | Antiserum will specifically react with a 34 KDa cdc2 protein from human, rat and mouse tissue. Cross reactivity with cdc2 from other species may also occur. |
| Reactivity: | Human,Mouse,Rat |
| Application image: |  |
| Application image note: | CDC2 polyclonal antibody (Cat # PAB9939) was used todetect human CDC2 by western blot in untreated (Contol) and drug treated lysates of MCF-7 cells. Lane 1-4 represents 3.1 uM, 6.2 uM, 12.5 uM and 25.0 uM genistein treatment of cells before lysates were prepared. Detection occurs using a 1 : 1,000 dilution. Although this antiserum detects non-specific bands at higher MW, a clear induction ofsignal is observed as the concentration of drug is increased. Personnel Communication, Xiao He Yang, University of Oklahoma Health Sciences Center. |
| Applications: | IP,ELISA,IHC,WB-Ce |
| Shipping condition: | Dry Ice |
| Publications: | Activation of cdc2 protein kinase during mitosis in human cells: cell cycle-dependent phosphorylation and subunit rearrangement.Draetta G, Beach D. Cell. 1988 Jul 1;54(1):17-26. |