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Data sheet of CAMK4 polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Bovine,Chicken,Chimpanzee,Dog,Frog,Human,Mouse,Rat |
| Host species | Rabbit |
| Applications | WB,ELISA,IHC-P |
More info about CAMK4 polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB9938 |
| Product name: | CAMK4 polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against synthetic peptide of CAMK4. |
| Gene id: | 814 |
| Gene name: | CAMK4 |
| Gene alias: | CaMK-GR|MGC36771 |
| Gene description: | calcium/calmodulin-dependent protein kinase IV |
| Immunogen: | A synthetic peptide corresponding to amino acids 305-323 of human CAMK4. |
| Protein accession: | Q16566(Human);P08414(Mouse);P13234(Rat) |
| Form: | Lyophilized |
| Recommend dilutions: | ELISA (1:5000-1:25000) Western Blot (1:500-1:2000) The optimal working dilution should be determined by the end user. |
| Storage buffer: | Lyophilized from 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide) |
| Storage instruction: | Store at 4°C on dry atmosphere. After reconstitution with 0.1 mL of deionized water, store at -20°C or lower. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Synthetic Peptide. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Human |
| Reactivity: | Bovine,Chicken,Chimpanzee,Dog,Frog,Human,Mouse,Rat |
| Application image: |  |
| Application image note: | Western blot using CAMK4 polyclonal antibody (Cat # PAB9938) shows detection of a band ~52 KDa corresponding to CAMK4 (arrowhead) in various preparations. Staining ofrat brain lysate is shown in lane 1. Jurkat cell lysate staining is shown in lane 2. Specific reactivity is blocked in both lysates when antibodyis preincubated with immunizing peptide (lanes 3 and 4 respectively). Approximately 35 ug of each lysate was separated by 4-20% SDS-PAGE and transferred onto nitrocellulose. CAMK4 was similarly detected on lysates from mouse brain (not shown). After blocking the membrane was probed with the primary antibody diluted to 1 : 1,000 for 2h at room temperature followed by washes and reaction with a 1 : 10,000 dilution of IRDyeâ„¢800 conjugated Gt-a-Rabbit IgG [H&L]MX for 45 min at room temperature. |
| Applications: | WB,ELISA,IHC-P |
| Shipping condition: | Dry Ice |
| Publications: | The cDNA sequence and characterization of the Ca2+/calmodulin-dependent protein kinase-Gr from human brain and thymus.Bland MM, Monroe RS, Ohmstede CA. Gene. 1994 May 16;142(2):191-7. |