Cart 0 Product Products (empty)
No products
To be determined Shipping
0,00 € Total
Prices are tax excluded
Product successfully added to your shopping cart
Quantity
Total
There are 0 items in your cart. There is 1 item in your cart.
Total products (tax excl.)
Total shipping (tax excl.) To be determined
Total (tax excl.)
Data sheet of ANAPC1 (phospho S377) polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Bovine,Dog,Human,Mouse,Rat |
| Host species | Rabbit |
| Applications | ELISA,WB-Ce,IHC-P |
More info about ANAPC1 (phospho S377) polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB9936 |
| Product name: | ANAPC1 (phospho S377) polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against synthetic phosphopeptide of ANAPC1. |
| Gene id: | 64682 |
| Gene name: | ANAPC1 |
| Gene alias: | APC1|MCPR|TSG24 |
| Gene description: | anaphase promoting complex subunit 1 |
| Immunogen: | Synthetic phosphopeptide corresponding to residues surrounding S377 of human ANAPC1. |
| Protein accession: | Q9H1A4;NP_073153 |
| Form: | Liquid |
| Recommend dilutions: | ELISA (1:2000-1:10000) Western Blot (1:200-1:1000) Immunohistochemistry (5.0 ug/mL) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide) |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Synthetic Peptide. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Human |
| Specificity: | This antibody is specific to phosphorylated human APC1 protein at the pS377 residue. |
| Reactivity: | Bovine,Dog,Human,Mouse,Rat |
| Application image: |  |
| Application image note: | Western blot using ANAPC1 (phospho S377) polyclonal antibody (Cat # PAB9936) shows detection of a band ~215 KDa corresponding to phosphorylated human ANAPC1 (arrowhead). Lane 1 shows lysate from asynchronous cells. Lane 2 shows lysate from cells treated with nocodazole. While some phosphorylated ANAPC1 is present in untreated cell, the amount of phosphorylated protein is increased in cell preparations arrested in mitosis. Each lane contains approximately 30 ug of HeLa whole cell lysates, separated by 4-8% SDS-PAGE followed by transfer to nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1 : 1,000 overnight at 4°C followed by washes and reaction with a 1 : 10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature. IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. |
| Applications: | ELISA,WB-Ce,IHC-P |
| Shipping condition: | Dry Ice |
| Publications: | Characterisation of the human APC1, the largest subunit of the anaphase-promoting complex.Jorgensen PM, Graslund S, Betz R, Stahl S, Larsson C, Hoog C. Gene. 2001 Jan 10;262(1-2):51-9. |