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Data sheet of AP2A1 polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Chicken,Chimpanzee,Dog,Human,Mouse,Orangutan,Rat |
| Host species | Goat |
| Applications | IP,ELISA,WB-Ce |
More info about AP2A1 polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB9935 |
| Product name: | AP2A1 polyclonal antibody |
| Product description: | Goat polyclonal antibody raised against synthetic peptide of AP2A1. |
| Gene id: | 160 |
| Gene name: | AP2A1 |
| Gene alias: | ADTAA|AP2-ALPHA|CLAPA1 |
| Gene description: | adaptor-related protein complex 2, alpha 1 subunit |
| Immunogen: | A synthetic peptide corresponding to amino acids 3-14 of human AP2A1. |
| Protein accession: | O95782;NP_055018(alpha1);NP_036437(alpha2) |
| Form: | Liquid |
| Recommend dilutions: | ELISA (1:5000-1:20000) Western Blot (1:500-1:2000) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide) |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Synthetic Peptide. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Goat |
| Antigen species / target species: | Human |
| Reactivity: | Chicken,Chimpanzee,Dog,Human,Mouse,Orangutan,Rat |
| Application image: |  |
| Application image note: | Western blot using AP2A1 polyclonal antibody (Cat # PAB9935) shows detection of a band just below 100 KDa correspond-ing to Human AP2A1 in a various preparations. Lane 1 - HeLa nuclear extract. Lane 2 - HeLa. Lane 3 - 293. Lane 4 - A-431. Lane 5 - Jurkat whole cell lysates. In lanes 6-10 the antibody was preincubated with 1 ug/mL of the immunizing peptide which effectively blocks the specific reactivity of this antibody with AP2A1. Approximately 20 ug of each lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1 : 500 dilution of AP2A1 polyclonal antibody. Detection occurred using a 1 : 5,000 dilution of HRP-labeled Rabbit anti-Goat IgG for 1 hour at room temperature. A chemiluminescence system was used for signal detection using a 60-sec exposure time. |
| Applications: | IP,ELISA,WB-Ce |
| Shipping condition: | Dry Ice |
| Publications: | Adaptins: the final recount.Boehm M, Bonifacino JS. Mol Biol Cell. 2001 Oct;12(10):2907-20. |