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Data sheet of AKT2 polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Human,Mouse,Rat |
| Host species | Rabbit |
| Applications | ELISA,WB-Ce,WB-Ti,WB-Tr |
More info about AKT2 polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB9933 |
| Product name: | AKT2 polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against synthetic peptide of AKT2. |
| Gene id: | 208 |
| Gene name: | AKT2 |
| Gene alias: | PKBB|PKBBETA|PRKBB|RAC-BETA |
| Gene description: | v-akt murine thymoma viral oncogene homolog 2 |
| Immunogen: | A synthetic peptide (conjugated with KLH) corresponding to amino acids 455-468 of human AKT2. |
| Immunogen sequence/protein sequence: | RYDSLGLLELDQRT |
| Form: | Liquid |
| Recommend dilutions: | ELISA (1:4000-1:16000) Western Blot (1:1000) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide) |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Synthetic Peptide. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Human |
| Specificity: | This antibody detects both unphosphorylated and phosphorylated forms of the protein. |
| Reactivity: | Human,Mouse,Rat |
| Application image: |  |
| Application image note: | Immunoblotting of AKT2 polyclonal antibody (Cat # PAB9933) detects both unphosphorylated and phosphorylated AKT2. As before approximately 20 mg/lane of crude HEK293 lysate was loaded for SDS-PAGE, separated and then transferred to nitrocellulose. The right lane contains lysate pretreated for 15 min with 25 &mciro;M LY294002 which affects the phosphorylation of endogenous AKT2, but has no effect on phosphorylation of the myristoylated construct. This antibody clearly detects both the phosphorylated (top arrow) and the non-phosphorylated (bottom arrow) forms of endogenous AKT2. |
| Applications: | ELISA,WB-Ce,WB-Ti,WB-Tr |
| Shipping condition: | Dry Ice |
| Publications: | Amplification of AKT2 in human pancreatic cells and inhibition of AKT2 expression and tumorigenicity by antisense RNA.Cheng JQ, Ruggeri B, Klein WM, Sonoda G, Altomare DA, Watson DK, Testa JR. Proc Natl Acad Sci U S A. 1996 Apr 16;93(8):3636-41. |