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Data sheet of AKT1 polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Chicken,Human,Mouse,Rat |
| Host species | Sheep |
| Applications | WB,ELISA |
More info about AKT1 polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB9932 |
| Product name: | AKT1 polyclonal antibody |
| Product description: | Sheep polyclonal antibody raised against synthetic peptide of AKT1. |
| Gene id: | 207 |
| Gene name: | AKT1 |
| Gene alias: | AKT|MGC99656|PKB|PKB-ALPHA|PRKBA|RAC|RAC-ALPHA |
| Gene description: | v-akt murine thymoma viral oncogene homolog 1 |
| Immunogen: | A synthetic peptide corresponding to internal region of human AKT1. |
| Protein accession: | NP_005154;P31749 |
| Form: | Liquid |
| Recommend dilutions: | ELISA (1:5000-1:20000) Western Blot (1:500-1:2000) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.1% sodium azide) |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Synthetic Peptide. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Sheep |
| Antigen species / target species: | Human |
| Reactivity: | Chicken,Human,Mouse,Rat |
| Application image: |  |
| Application image note: | Western blot using AKT1 polyclonal antibody (Cat # PAB9932) shows detection of endogenous protein in whole cell extracts from H2O2 treated MCF-7 cells (Lane 2). Recombinant AKT1 is detected in lane 3. Lanes 1 and 4 contain wcl from HeLa and A-431 cells, respectively. Specific band staining is completely blocked when antibody is pre-incubated with the immunizing peptide (data not shown). The band at ~55-60 KDa, indicated by the arrowhead, corresponds to the expected molecular weight of AKT1. Primary antibody was diluted 1 : 300 in 1% BLOTTO and reacted with the membrane overnight at 4°C. IRDYE800™ conjugated Dnky-a-Sheep IgG was used at a 1 : 10,000 dilution for 45 min at room temperature. |
| Applications: | WB,ELISA |
| Shipping condition: | Dry Ice |
| Publications: | Regulation of mammalian target of rapamycin activity in PTEN-inactive prostate cancer cells by I kappa B kinase alpha.Dan HC, Adli M, Baldwin AS. Cancer Res. 2007 Jul 1;67(13):6263-9. |