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Data sheet of PTENP1 polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Chimpanzee,Dog,Human,Macaque,Mouse,Opossum,Pufferfishes,Rat |
| Host species | Rabbit |
| Applications | ELISA,WB-Ce |
More info about PTENP1 polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB9923 |
| Product name: | PTENP1 polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against partial recombinant PTENP1. |
| Gene id: | 11191 |
| Gene name: | PTENP1 |
| Gene alias: | PTEN-rs|PTEN2|PTH2|psiPTEN |
| Gene description: | phosphatase and tensin homolog pseudogene 1 |
| Immunogen: | Recombinant protein corresponding to N-terminus of human PTENP1. |
| Protein accession: | O43460;NP_000305;AAH38293 |
| Form: | Liquid |
| Recommend dilutions: | ELISA (1:160000) Western Blot (1:500-1:2000) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide) |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Recombinant Protein. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Human |
| Reactivity: | Chimpanzee,Dog,Human,Macaque,Mouse,Opossum,Pufferfishes,Rat |
| Application image: |  |
| Application image note: | Western blot using PTENP1 polyclonal antibody (Cat # PAB9923) shows detection of endogenous PTENP1 in whole cell lysates from human derived cell lines HeLa (Lane 4), HEK293 (Lane 5) and MCF-7 (Lane 6). The band at ~55 KDa (arrowhead) corresponds to PTENP1. Lanes 1-3 were show the results of staining after the antibody was first pre-incubated with the immunizing peptide. The identity of lower molecular weight bands in lane 4 is unknown. Briefly, each lane contains approximately 35 ug of lysate. Primary antibody was used at a 1 : 500 dilution in 5% BLOTTO in PBS reacted overnight at 4°C. The membrane was washed and reacted with a 1 : 10,000 dilution of IRDye800™ conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red). IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. |
| Applications: | ELISA,WB-Ce |
| Shipping condition: | Dry Ice |
| Publications: | Alterations of PTEN/MMAC1, a candidate tumor suppressor gene, and its homologue, PTH2, in small cell lung cancer cell lines.Kim SK, Su LK, Oh Y, Kemp BL, Hong WK, Mao L. Oncogene. 1998 Jan 8;16(1):89-93. |