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Data sheet of His2Av (phospho S137) polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Fruit fly |
| Host species | Rabbit |
| Applications | ELISA,WB-Ti |
More info about His2Av (phospho S137) polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB9920 |
| Product name: | His2Av (phospho S137) polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against synthetic phsphopeptide of His2Av. |
| Gene id: | 43229 |
| Gene name: | His2Av |
| Gene alias: | CG5499|H2A.X|H2A.Z|H2AV|H2AX|H2AZ|H2AvD|His2AvD|l(3)05146|l(3)810|l(3)97Dd|l(3)L1602 |
| Gene description: | Histone H2A variant |
| Immunogen: | Synthetic phosphopeptide corresponding to residues surrounding S137 of drosophila His2Av. |
| Protein accession: | S08118;P08985;NP_524519 |
| Form: | Liquid |
| Recommend dilutions: | ELISA (1:4000-1:16000) Western Blot (1:400-1:1600) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide) |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Synthetic Peptide. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Fruit fly |
| Specificity: | Reactivity occurs against Drosophila H2AvD pS137 protein and This antibody is specific to the phosphorylated form of the protein. Reactivity with non-phosphorylated Drosophila H2AvD is minimal by ELISA. |
| Reactivity: | Fruit fly |
| Application image: |  |
| Application image note: | Western blot using His2Av (phospho S137) polyclonal antibody (Cat # PAB9920) shows detection of aband at ~ 15 KDa corresponding to Phospho-His2Av S137 (Lane 2 arrow-head). Lanes contain either mock-irradiated (Lane 1) or 4000-RAD gamma irradiated (Lane 2) Drosophila melanogaster (3rd instar) larvae brainWC lysate separated on by SDS-PAGE and transferred to nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1 : 500. Washes and reaction with secondary antibody followed incubation. Use HRP conjugated Gt-a-Rabbit IgG [H&L] MX and ECL for detection. Personal Communication. Yikang Rong, NIH, CCR, Bethesda, MD. |
| Applications: | ELISA,WB-Ti |
| Shipping condition: | Dry Ice |
| Publications: | DNA double-strand break-induced phosphorylation of Drosophila histone variant H2Av helps prevent radiation-induced apoptosis.Madigan JP, Chotkowski HL, Glaser RL. Nucleic Acids Res. 2002 Sep 1;30(17):3698-705. |