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Data sheet of Dynamin (phospho S774) polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Bovine,Chicken,Dog,Human,Mouse,Primates,Rat |
| Host species | Sheep |
| Applications | IHC,WB-Ti,IF |
More info about Dynamin (phospho S774) polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB9697 |
| Product name: | Dynamin (phospho S774) polyclonal antibody |
| Product description: | Sheep polyclonal antibody raised against synthetic phosphopeptide of dynamin. |
| Immunogen: | Synthetic phosphopeptide corresponding to residues surrounding S774 of rat dynamin. |
| Protein accession: | P21575 |
| Form: | Liquid |
| Recommend dilutions: | Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:1000) Immunofluorescence (1:1000) Western Blot (1:1000) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In 10 mM HEPES, 150 mM NaCl, pH 7.5 (50% glycerol, 10% BSA) |
| Storage instruction: | Store at -80°C. For short term storage, store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Synthetic Peptide. |
| Product type: | Primary antibodies |
| Host species: | Sheep |
| Antigen species / target species: | Rat |
| Reactivity: | Bovine,Chicken,Dog,Human,Mouse,Primates,Rat |
| Application image: |  |
| Application image note: | Western blot of rat hippocampal lysate stimulated with forskolin showing specific immunolabeling of the ~95k Dynamin phosphorylated at Ser774 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lambda-Ptase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the Dynamin (phospho S774) polyclonal antibody (Cat # PAB9697). The immunolabeling is completely eliminated by treatment with lambda-Ptase. |
| Applications: | IHC,WB-Ti,IF |
| Shipping condition: | Dry Ice |
| Publications: | Sweeping model of dynamin activity. Visualization of coupling between exocytosis and endocytosis under an evanescent wave microscope with green fluorescent proteins.Tsuboi T, Terakawa S, Scalettar BA, Fantus C, Roder J, Jeromin A. J Biol Chem. 2002 May 3;277(18):15957-61. Epub 2002 Feb 20. |