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Data sheet of Cdc2 (phospho Y15) polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Clawed frog,Human,Mouse,Rat,Zebra fish |
| Host species | Rabbit |
| Applications | WB-Ce |
More info about Cdc2 (phospho Y15) polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB9677 |
| Product name: | Cdc2 (phospho Y15) polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against synthetic phosphopeptide of Cdc2. |
| Gene id: | 54237 |
| Gene name: | Cdc2 |
| Gene alias: | Cdc2a |
| Gene description: | cell division cycle 2, G1 to S and G2 to M |
| Immunogen: | Synthetic phosphopeptide corresponding to residues surrounding Y15 of rat Cdc2. |
| Protein accession: | Q5XI98 |
| Form: | Liquid |
| Recommend dilutions: | Western Blot (1:1000) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In 10 mM HEPES, 150 mM NaCl, pH 7.5 (50% glycerol, 10% BSA) |
| Storage instruction: | Store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Synthetic Peptide. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Rat |
| Reactivity: | Clawed frog,Human,Mouse,Rat,Zebra fish |
| Application image: |  |
| Application image note: | Western blot of human T-47D cells showing specific immunolabeling of the ~34k Cdc2 phosphorylated at Tyr15 (Control, lane 1). Treatment with EGF (30 ng per ml for 30 min) caused dephosphorylation of the Tyr15 on cdc2 (Lane 2). The phosphospecificity of this labeling is also shown in the third and fourth lanes (lambda-phosphatase: lambda-Ptase). These blots are identical to the control except that they were incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the Phospho-Cdc2 Y15. The immunolabeling is completely eliminated by treatment with lambda-Ptase. |
| Applications: | WB-Ce |
| Shipping condition: | Dry Ice |
| Publications: | Cdc2 phosphorylation of nucleolin demarcates mitotic stages and Alzheimer's disease pathology.Dranovsky A, Vincent I, Gregori L, Schwarzman A, Colflesh D, Enghild J, Strittmatter W, Davies P, Goldgaber D. Neurobiol Aging. 2001 Jul-Aug;22(4):517-28. |