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Data sheet of Grin2b (phospho Y1472) polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Bovine,Chicken,Dog,Human,Mouse,Primates,Rat,Zebra fish |
| Host species | Rabbit |
| Applications | WB-Ti |
More info about Grin2b (phospho Y1472) polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB9673 |
| Product name: | Grin2b (phospho Y1472) polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against synthetic phosphopeptide of Grin2b. |
| Gene id: | 24410 |
| Gene name: | Grin2b |
| Gene alias: | - |
| Gene description: | glutamate receptor, ionotropic, N-methyl D-aspartate 2B |
| Immunogen: | Synthetic phosphopeptide corresponding to residues surrounding Y1472 of rat Grin2b. |
| Protein accession: | Q00960 |
| Form: | Liquid |
| Recommend dilutions: | Western Blot (1:1000) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In 10 mM HEPES, 150 mM NaCl, pH 7.5 (50% glycerol, 10% BSA) |
| Storage instruction: | Store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Synthetic Peptide. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Rat |
| Specificity: | Specific for the ~180k NMDAR NR2B-subunit protein phosphorylated at Tyr1472 in Western blots. This antibody also labels proteins of ~65k and ~115k. Immunolabeling is completely blocked by blocked by either l-Ptase or by the phosphopeptide used as the antigen but not by the corresponding dephosphopeptide. |
| Reactivity: | Bovine,Chicken,Dog,Human,Mouse,Primates,Rat,Zebra fish |
| Application image: |  |
| Application image note: | Western blot of rat hippocampal lysate showing specific immunolabeling of the ~180k Grin2b subunit of the NMDAR phosphorylated at Tyr1472 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lambda-Ptase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the Grin2b (phospho Y1472) polyclonal antibody (Cat # PAB9673). The immunolabeling is completely eliminated by treatment with lambda-Ptase. |
| Applications: | WB-Ti |
| Shipping condition: | Dry Ice |
| Publications: | Trafficking of NMDA receptors.Wenthold RJ, Prybylowski K, Standley S, Sans N, Petralia RS. Annu Rev Pharmacol Toxicol. 2003;43:335-58. Epub 2002 Jan 10. |