Brand | Abnova |
Product type | Primary antibodies |
Reactivity | Bovine,Chicken,Clawed frog,Dog,Human,Mouse,Primates,Rat,Zebra fish |
Host species | Rabbit |
Applications | WB-Ce |
Brand: | Abnova |
Reference: | PAB9666 |
Product name: | ERK/MAPK (phospho T202/Y204) polyclonal antibody |
Product description: | Rabbit polyclonal antibody raised against synthetic phosphopeptide of ERK/MAPK. |
Immunogen: | Synthetic phosphopeptide corresponding to residues surrounding T202/Y204 of rat ERK/MAPK. |
Protein accession: | P63086 |
Form: | Liquid |
Recommend dilutions: | Western Blot (1:1000) The optimal working dilution should be determined by the end user. |
Storage buffer: | In 10 mM HEPES, 150 mM NaCl, pH 7.5 (50% glycerol, 10% BSA) |
Storage instruction: | Store at -20°C. Aliquot to avoid repeated freezing and thawing. |
Quality control testing: | Antibody Reactive Against Synthetic Peptide. |
Product type: | Primary antibodies |
Host species: | Rabbit |
Antigen species / target species: | Rat |
Reactivity: | Bovine,Chicken,Clawed frog,Dog,Human,Mouse,Primates,Rat,Zebra fish |
Application image: | ![]() |
Application image note: | Western blot of human T-47D cell lysates showing specific immunolabeling of ~42-44k ERK/MAPK protein phosphorylated at Thr202 and Tyr204 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lambda-Ptase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the Anti-Thr202/Tyr204 ERK/MAPK. The immunolabeling is completely eliminated by treatment with lambda-Ptase. |
Applications: | WB-Ce |
Shipping condition: | Dry Ice |
Publications: | Molecular psychology: roles for the ERK MAP kinase cascade in memory.Adams JP, Sweatt JD. Annu Rev Pharmacol Toxicol. 2002;42:135-63. |