Brand | Abnova |
Product type | Primary antibodies |
Reactivity | Bovine,Clawed frog,Human,Mouse,Rat,Zebra fish |
Host species | Rabbit |
Applications | WB-Ti |
Brand: | Abnova |
Reference: | PAB9664 |
Product name: | Syn1 (phospho S9) polyclonal antibody |
Product description: | Rabbit polyclonal antibody raised against synthetic phosphopeptide of Syn1. |
Gene id: | 24949 |
Gene name: | Syn1 |
Gene alias: | - |
Gene description: | synapsin I |
Immunogen: | Synthetic phosphopeptide corresponding to residues surrounding S9 of rat Syn1. |
Protein accession: | P17599 |
Form: | Liquid |
Recommend dilutions: | Western Blot (1:1000) The optimal working dilution should be determined by the end user. |
Storage buffer: | In 10 mM HEPES, 150 mM NaCl, pH 7.5 (50% glycerol, 10% BSA) |
Storage instruction: | Store at -20°C. Aliquot to avoid repeated freezing and thawing. |
Quality control testing: | Antibody Reactive Against Synthetic Peptide. |
Product type: | Primary antibodies |
Host species: | Rabbit |
Antigen species / target species: | Rat |
Specificity: | This antibody also weakly labels the ~55k synapsin II protein which has a similar phosphorylation site to that of Ser9 on synapsin I. |
Reactivity: | Bovine,Clawed frog,Human,Mouse,Rat,Zebra fish |
Application image: | ![]() |
Application image note: | Western blot of rat cortex lysate showing specific immunolabeling of the ~78k Syn1 phosphorylated at Ser9 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lambda-Ptase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the Syn1 (phospho S9) polyclonal antibody (Cat # PAB9664). The immunolabeling is completely eliminated by treatment with lambda-Ptase. |
Applications: | WB-Ti |
Shipping condition: | Dry Ice |
Publications: | Opposing changes in phosphorylation of specific sites in synapsin I during Ca2+-dependent glutamate release in isolated nerve terminals.Jovanovic JN, Sihra TS, Nairn AC, Hemmings HC Jr, Greengard P, Czernik AJ. J Neurosci. 2001 Oct 15;21(20):7944-53. |