Raf1 (phospho S301) polyclonal antibody View larger

Raf1 (phospho S301) polyclonal antibody

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Data sheet of Raf1 (phospho S301) polyclonal antibody

BrandAbnova
Product typePrimary antibodies
ReactivityBovine,Chicken,Clawed frog,Dog,Human,Mouse,Primates,Rat
Host speciesRabbit
ApplicationsWB-Ce

More info about Raf1 (phospho S301) polyclonal antibody

Brand: Abnova
Reference: PAB9647
Product name: Raf1 (phospho S301) polyclonal antibody
Product description: Rabbit polyclonal antibody raised against synthetic phosphopeptide of Raf1.
Gene id: 24703
Gene name: Raf1
Gene alias: -
Gene description: v-raf-leukemia viral oncogene 1
Immunogen: Synthetic phosphopeptide corresponding to residues surrounding S301 of rat Raf1.
Protein accession: P11345
Form: Liquid
Recommend dilutions: Western Blot (1:1000)
The optimal working dilution should be determined by the end user.
Storage buffer: In 10 mM HEPES, 150 mM NaCl, pH 7.5 (50% glycerol, 10% BSA)
Storage instruction: Store at -20°C.
Aliquot to avoid repeated freezing and thawing.
Quality control testing: Antibody Reactive Against Synthetic Peptide.
Product type: Primary antibodies
Host species: Rabbit
Antigen species / target species: Rat
Reactivity: Bovine,Chicken,Clawed frog,Dog,Human,Mouse,Primates,Rat
Application image: PAB9647-46-6-1.jpg
Application image note: Western blot of UV treated human Jurkat cell lysate showing specific immunolabeling of the ~74k Raf1 protein (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lambda-Ptase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the Raf1 (phospho S301) polyclonal antibody (Cat # PAB9647). The immunolabeling of Raf1 is completely eliminated by treatment with lambda-Ptase.
Applications: WB-Ce
Shipping condition: Dry Ice
Publications: Untying the regulation of the Raf-1 kinase.Dhillon AS, Kolch W.
Arch Biochem Biophys. 2002 Aug 1;404(1):3-9.

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