Brand | Abnova |
Product type | Primary antibodies |
Reactivity | Bovine,Chicken,Clawed frog,Dog,Human,Mouse,Primates,Rat |
Host species | Rabbit |
Applications | WB-Ce |
Brand: | Abnova |
Reference: | PAB9647 |
Product name: | Raf1 (phospho S301) polyclonal antibody |
Product description: | Rabbit polyclonal antibody raised against synthetic phosphopeptide of Raf1. |
Gene id: | 24703 |
Gene name: | Raf1 |
Gene alias: | - |
Gene description: | v-raf-leukemia viral oncogene 1 |
Immunogen: | Synthetic phosphopeptide corresponding to residues surrounding S301 of rat Raf1. |
Protein accession: | P11345 |
Form: | Liquid |
Recommend dilutions: | Western Blot (1:1000) The optimal working dilution should be determined by the end user. |
Storage buffer: | In 10 mM HEPES, 150 mM NaCl, pH 7.5 (50% glycerol, 10% BSA) |
Storage instruction: | Store at -20°C. Aliquot to avoid repeated freezing and thawing. |
Quality control testing: | Antibody Reactive Against Synthetic Peptide. |
Product type: | Primary antibodies |
Host species: | Rabbit |
Antigen species / target species: | Rat |
Reactivity: | Bovine,Chicken,Clawed frog,Dog,Human,Mouse,Primates,Rat |
Application image: | ![]() |
Application image note: | Western blot of UV treated human Jurkat cell lysate showing specific immunolabeling of the ~74k Raf1 protein (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lambda-Ptase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the Raf1 (phospho S301) polyclonal antibody (Cat # PAB9647). The immunolabeling of Raf1 is completely eliminated by treatment with lambda-Ptase. |
Applications: | WB-Ce |
Shipping condition: | Dry Ice |
Publications: | Untying the regulation of the Raf-1 kinase.Dhillon AS, Kolch W. Arch Biochem Biophys. 2002 Aug 1;404(1):3-9. |