Brand | Abnova |
Product type | Primary antibodies |
Reactivity | Bovine,Dog,Human,Mouse,Primates,Rat |
Host species | Rabbit |
Applications | WB-Re |
Brand: | Abnova |
Reference: | PAB9608 |
Product name: | MAP2K1 (phospho T292) polyclonal antibody |
Product description: | Rabbit polyclonal antibody raised against synthetic phosphopeptide of MAP2K1. |
Gene id: | 5604 |
Gene name: | MAP2K1 |
Gene alias: | MAPKK1|MEK1|MKK1|PRKMK1 |
Gene description: | mitogen-activated protein kinase kinase 1 |
Immunogen: | Synthetic phosphopeptide corresponding to residues surrounding T292 of human MAP2K1. |
Protein accession: | Q02750 |
Form: | Liquid |
Recommend dilutions: | Western Blot (1:1000) The optimal working dilution should be determined by the end user. |
Storage buffer: | In 10 mM HEPES, 150 mM NaCl, pH 7.5 (50% glycerol, 10% BSA) |
Storage instruction: | Store at -20°C. Aliquot to avoid repeated freezing and thawing. |
Quality control testing: | Antibody Reactive Against Synthetic Peptide. |
Product type: | Primary antibodies |
Host species: | Rabbit |
Antigen species / target species: | Human |
Reactivity: | Bovine,Dog,Human,Mouse,Primates,Rat |
Application image: | ![]() |
Application image note: | Western blot of recombinant wild type and mutant MAP2K1 showing immunolabeling of the ~45k MAP2K1 protein phosphorylated at Thr292. Lanes 1 and 2 are WT MAP2K1 and Lanes 3 and 4 are mutant MAP2K1 (T292A). MAP kinase was coexpressed in the samples run in Lanes 2 and 4. |
Applications: | WB-Re |
Shipping condition: | Dry Ice |
Publications: | Mitogen-activated protein (MAP) kinase phosphorylation of MAP kinase kinase: determination of phosphorylation sites by mass spectrometry and site-directed mutagenesis.Mansour SJ, Resing KA, Candi JM, Hermann AS, Gloor JW, Herskind KR, Wartmann M, Davis RJ, Ahn NG. J Biochem. 1994 Aug;116(2):304-14. |