NFKBIA (phospho Y42) polyclonal antibody View larger

NFKBIA (phospho Y42) polyclonal antibody

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Data sheet of NFKBIA (phospho Y42) polyclonal antibody

BrandAbnova
Product typePrimary antibodies
ReactivityHuman,Mouse,Rat
Host speciesRabbit
ApplicationsIP,ELISA,WB-Ce

More info about NFKBIA (phospho Y42) polyclonal antibody

Brand: Abnova
Reference: PAB7922
Product name: NFKBIA (phospho Y42) polyclonal antibody
Product description: Rabbit polyclonal antibody raised against synthetic phosphopeptide of NFKBIA.
Gene id: 4792
Gene name: NFKBIA
Gene alias: IKBA|MAD-3|NFKBI
Gene description: nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha
Immunogen: Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding Y42 of human NFKBIA.
Form: Liquid
Recommend dilutions: ELISA (1:2000)
Immunoprecipitation (5-10 ul)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.
Storage buffer: In PBS (50% glycerol, 1 mg/mL BSA, 0.05% sodium azide)
Storage instruction: Store at -20°C.
Aliquot to avoid repeated freezing and thawing.
Quality control testing: Antibody Reactive Against Synthetic Peptide.
Note: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Product type: Primary antibodies
Host species: Rabbit
Antigen species / target species: Human
Specificity: This peptide sequence has low homology to other IkB proteins, but does have some homology to unrelated proteins that may have a conserved tyrosine phosphorylation motif.
Reactivity: Human,Mouse,Rat
Application image: PAB7922-46-4-1.jpg
Application image note: Western blot analysis of A-431 cells treated with pervanadate (1 mM) for 30 min. Blots were probed with NFKBIA (phospho Y42) polyclonal antibody (Cat # PAB7922). The latter antibodies were used in the presence of no blocking peptide (lane 1), phospho-NFKBIA (Tyr-42) peptide (lane 2), phospho-NFKBIA (Tyr-305) peptide (lane 3), or BSA conjugated to phospho-tyrosine (lane 4). Peptides and BSA-pTyr were used at 1 mg/ml.
Applications: IP,ELISA,WB-Ce
Shipping condition: Dry Ice
Publications: Regulation of COX-2 mediated signaling by alpha3 type IV noncollagenous domain in tumor angiogenesis.Boosani CS, Mannam AP, Cosgrove D, Silva R, Hodivala-Dilke KM, Keshamouni VG, Sudhakar A.
Blood. 2007 Aug 15;110(4):1168-77. Epub 2007 Apr 10.

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