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Data sheet of NUP85 polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Human |
| Host species | Goat |
| Applications | ELISA,WB-Ce |
More info about NUP85 polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB6540 |
| Product name: | NUP85 polyclonal antibody |
| Product description: | Goat polyclonal antibody raised against synthetic peptide of NUP85. |
| Gene id: | 79902 |
| Gene name: | NUP85 |
| Gene alias: | FLJ12549|Nup75 |
| Gene description: | nucleoporin 85kDa |
| Immunogen: | A synthetic peptide corresponding to C-terminus of human NUP85. |
| Immunogen sequence/protein sequence: | C-ARAIIREGSLEGS |
| Protein accession: | NP_079120.1 |
| Form: | Liquid |
| Concentration: | 0.5 mg/mL |
| Recommend dilutions: | ELISA (1:32000) Western Blot (1-3 ug/mL) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide) |
| Storage instruction: | Store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Goat |
| Antigen species / target species: | Human |
| Specificity: | In contrast to other anti-Pericentrin antibodies available, this antibody is NOT expected to recognize Kendrin (also known as Pericentrin 2). This may explain why it recognizes a 75 KDa band (the expected size according to NP_079120) and not a 220 KDa band (as reported for other anti-pericentrin antibodies). |
| Reactivity: | Human |
| Application image: |  |
| Application image note: | NUP85 polyclonal antibody (Cat # PAB6540) (1 ug/mL) staining of HeLa lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. |
| Applications: | ELISA,WB-Ce |
| Shipping condition: | Dry Ice |
| Publications: | Pericentrin, a highly conserved centrosome protein involved in microtubule organization.Doxsey SJ, Stein P, Evans L, Calarco PD, Kirschner M. Cell. 1994 Feb 25;76(4):639-50. |