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Data sheet of ARAF polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Human |
| Host species | Rabbit |
| Applications | WB-Ce,IHC-P |
More info about ARAF polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB3478 |
| Product name: | ARAF polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against synthetic peptide of ARAF. |
| Gene id: | 369 |
| Gene name: | ARAF |
| Gene alias: | A-RAF|ARAF1|PKS2|RAFA1 |
| Gene description: | v-raf murine sarcoma 3611 viral oncogene homolog |
| Immunogen: | A synthetic peptide (conjugated with KLH) corresponding to internal region of human ARAF. |
| Protein accession: | P10398 |
| Form: | Liquid |
| Recommend dilutions: | Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-100) Western Blot (1:1000) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In PBS (0.09% sodium azide) |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Human |
| Reactivity: | Human |
| Application image: |  |
| Application image note: | Formalin-fixed and paraffin-embedded human cancer tissue reacted with ARAF polyclonal antibody (Cat # PAB3478) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma. |
| Applications: | WB-Ce,IHC-P |
| Shipping condition: | Dry Ice |
| Publications: | Selective activation of MEK1 but not MEK2 by A-Raf from epidermal growth factor-stimulated Hela cells.Wu X, Noh SJ, Zhou G, Dixon JE, Guan KL. J Biol Chem. 1996 Feb 9;271(6):3265-71. |