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Data sheet of SMAD3 (phospho T179) polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Human |
| Host species | Rabbit |
| Applications | ELISA,IHC,WB-Ce |
More info about SMAD3 (phospho T179) polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB19947 |
| Product name: | SMAD3 (phospho T179) polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against synthetic phosphopeptide of SMAD3. |
| Gene id: | 4088 |
| Gene name: | SMAD3 |
| Gene alias: | DKFZp586N0721|DKFZp686J10186|HSPC193|HsT17436|JV15-2|MADH3|MGC60396 |
| Gene description: | SMAD family member 3 |
| Immunogen: | Synthetic phosphopeptide corresponding to residues surrounding T179 of human SMAD3. |
| Form: | Liquid |
| Recommend dilutions: | ELISA (1:15000-1:75000) Western Blot (1:1000) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide) |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Human |
| Specificity: | This affinity-purified antibody is directed against the phosphorylated form of human Smad3 protein at the pT179 residue. The product was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross adsorbed against the non-phosphorylated form of the immunizing peptide. Reactivity occurs against human Smad3 pT179 protein and the antibody is specific for the phosphorylated form of the protein. Reactivity with non-phosphorylated human Smad3 is minimal by ELISA and western blot. Expect reactivity against phosphorylated Smad2. Reactivity against other phosphorylated Smad family members is not known. A BLAST analysis was used to suggest cross reactivity with Smad3 from human, mouse, rat, pig, dog, and marmoset based on 100% sequence homology with the immunogen. Reactivity against homologues from other sources is not known. |
| Reactivity: | Human |
| Application image: |  |
| Application image note: | Western blot analysis of mouse mammary epithelial cell untreated (Lane 1) or treated with TGF-beta (Lane 2) by using SMAD3 (phospho T179) polyclonal antibody (Cat # PAB19947). |
| Applications: | ELISA,IHC,WB-Ce |
| Shipping condition: | Dry Ice |