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Data sheet of Myb (phospho T486) polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Mouse |
| Host species | Rabbit |
| Applications | ELISA,WB-Ce |
More info about Myb (phospho T486) polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB19946 |
| Product name: | Myb (phospho T486) polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against synthetic phosphopeptide of Myb. |
| Gene id: | 17863 |
| Gene name: | Myb |
| Gene alias: | AI550390|M16449|MGC18531|c-myb |
| Gene description: | myeloblastosis oncogene |
| Immunogen: | Synthetic phosphopeptide corresponding to residues surrounding T486 of mouse Myb. |
| Form: | Liquid |
| Recommend dilutions: | ELISA (1:5000-1:16000) Western Blot (1:2500) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide) |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Mouse |
| Specificity: | Reactivity occurs against mouse c-Myb pT486 protein and the antibody is specific for the phosphorylated form of the protein. This antibody reacts with endogenous c-Myb protein and also SUMOylated overexpressed c-Myb protein in transfected Cos7 cells. Reactivity with non-phosphorylated mouse c-Myb is minimal by ELISA and western blot. A BLAST analysis was used to suggest reactivity with c-Myb from mouse based on a 100% homology with the immunizing sequence. Expect reactivity with c-Myb from rat, pig, human, horse, platypus, opossum, orangutan, spider monkey, bonobo, gorilla, and chimpanzee; with a v-myb myeloblastosis viral oncogene homolog, all isoforms (also avian version) from human; and with a protooncogene c-myb, and a v-myb myeloblastosis viral oncogene homolog (avian), both from cattle, all based on a 93% homology with the immunizing sequence. Cross-reactivity with c-Myb from other sources has not been determined. |
| Reactivity: | Mouse |
| Application image: |  |
| Application image note: | Western blot analysis of COS7 cell untreated (Lane 1) or treated with okadaic acid (Lane 2) by using Myb (phospho T486) polyclonal antibody (Cat # PAB19946) at 1:2500 dilution. |
| Applications: | ELISA,WB-Ce |
| Shipping condition: | Dry Ice |