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UBE2F polyclonal antibody

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Data sheet of UBE2F polyclonal antibody

BrandAbnova
Product typePrimary antibodies
ReactivityHuman,Mouse
Host speciesGoat
ApplicationsELISA,WB-Ti,IHC-P

More info about UBE2F polyclonal antibody

Brand: Abnova
Reference: PAB17034
Product name: UBE2F polyclonal antibody
Product description: Goat polyclonal antibody raised against synthetic peptide of UBE2F.
Gene id: 140739
Gene name: UBE2F
Gene alias: MGC18120|NCE2
Gene description: ubiquitin-conjugating enzyme E2F (putative)
Immunogen: A synthetic peptide corresponding to C terminus of human UBE2F.
Immunogen sequence/protein sequence: C-HLRDKEDFRNKVDD
Protein accession: NP_542409.1
Form: Liquid
Concentration: 0.5 mg/mL
Recommend dilutions: ELISA (1:32000)
Western Blot (0.05-0.2 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (2.5-3.8 ug/mL)
The optimal working dilution should be determined by the end user.
Storage buffer: In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage instruction: Store at -20°C.
Aliquot to avoid repeated freezing and thawing.
Note: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Product type: Primary antibodies
Host species: Goat
Antigen species / target species: Human
Reactivity: Human,Mouse
Application image: PAB17034-47-C2-1.jpg
Application image note: UBE2F polyclonal antibody (Cat # PAB17034) (0.05 ug/mL) staining of mouse liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Applications: ELISA,WB-Ti,IHC-P
Shipping condition: Dry Ice
Publications: E2-RING expansion of the NEDD8 cascade confers specificity to cullin modification.Huang DT, Ayrault O, Hunt HW, Taherbhoy AM, Duda DM, Scott DC, Borg LA, Neale G, Murray PJ, Roussel MF, Schulman BA.
Mol Cell. 2009 Feb 27;33(4):483-95.

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