Brand | Abnova |
Product type | Primary antibodies |
Reactivity | Human,Mouse,Rat |
Host species | Rabbit |
Applications | ELISA,WB-Ce,IHC-P |
Brand: | Abnova |
Reference: | PAB16949 |
Product name: | ERK1/2 (phospho T202/Y204) polyclonal antibody |
Product description: | Rabbit polyclonal antibody raised against synthetic phosphopeptide of ERK1/2. |
Immunogen: | Synthetic phosphopeptide corresponding to residues surrounding T202/Y204 of human ERK1/2. |
Protein accession: | P27361 |
Form: | Liquid |
Recommend dilutions: | Western Blot (1:500-1:1000) Immunohistochemistry (1:50-1:100) ELISA (1:4000) The optimal working dilution should be determined by the end user. |
Storage buffer: | In 20 mM PBS, 0.15 M NaCl, pH 7.2 (0.01% sodium azide) |
Storage instruction: | Store at 4°C for three months. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. |
Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
Product type: | Primary antibodies |
Host species: | Rabbit |
Antigen species / target species: | Human |
Specificity: | This antibody detects endogenous levels of ERK1/2 only when phosphorylated at Thr202/Tyr204. |
Reactivity: | Human,Mouse,Rat |
Application image: | ![]() |
Application image note: | Western blot analysis of extracts from Jurkat cells treated with PMA. Lane 1 : Using ERK1/2 (phospho T202/Y204) polyclonal antibody (Cat # PAB16949). Lane 2 : Using the same antibody preincubated with synthesized peptide. |
Applications: | ELISA,WB-Ce,IHC-P |
Shipping condition: | Dry Ice |
Publications: | Differential regulation of MAP kinase signalling by dual-specificity protein phosphatases.Owens DM, Keyse SM. Oncogene. 2007 May 14;26(22):3203-13. |