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Data sheet of Aqp2 (phospho S264) polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Bovine,Chicken,Dog,Human,Mouse,Non-Human Primates,Rat |
| Host species | Rabbit |
| Applications | WB-Ti |
More info about Aqp2 (phospho S264) polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB16935 |
| Product name: | Aqp2 (phospho S264) polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against synthetic phosphopeptide of Aqp2. |
| Gene id: | 25386 |
| Gene name: | Aqp2 |
| Gene alias: | AQP-2|MGC156502|aquaporin-2 |
| Gene description: | aquaporin 2 (collecting duct) |
| Immunogen: | Synthetic phosphopeptide corresponding to residues surrounding S264 of rat Aqp2. |
| Protein accession: | P34080 |
| Form: | Liquid |
| Recommend dilutions: | Western Blot (1:1000) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In 10 mM HEPES, 150 mM NaCl, pH 7.5 (50% glycerol, 0.1 mg/mL BSA) |
| Storage instruction: | For long term store at -80°C is recommended, but shorter term storage at -20°C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50 % glycerol. Aliquot to avoid repeated freezing and thawing. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Rat |
| Specificity: | This antibody has been directly tested for reactivity in Western blots with rat tissue. It is anticipated that This antibody will react with canine, bovine, chicken, human, mouse and non-human primate tissues based on the fact that these species have 100% homology with the amino acid sequence used as antigen. specific to the ~29k AQP2 protein phosphorylated at Ser264. Also recognizes the glycosylated form of AQP2 at ~ 37k. Immunolabeling of the AQP2 band is blocked by preadsorption with the phospho-peptide used as antigen but not by the corresponding dephospho-peptide. |
| Reactivity: | Bovine,Chicken,Dog,Human,Mouse,Non-Human Primates,Rat |
| Application image: |  |
| Application image note: | Western blot of vasopressin (AVP, lane 2) treated rat kidney lysate and control (Lane 1) showing specific immunolabeling of the ~ 29k and 37k glycosylated form of the Aqp2 (phospho S264) polyclonal antibody (Cat ID # PAB16935). |
| Applications: | WB-Ti |
| Shipping condition: | Dry Ice |
| Publications: | Dynamics of aquaporin-2 serine-261 phosphorylation in response to short-term vasopressin treatment in collecting duct.Hoffert JD, Nielsen J, Yu MJ, Pisitkun T, Schleicher SM, Nielsen S, Knepper MA. Am J Physiol Renal Physiol. 2007 Feb;292(2):F691-700. Epub 2006 Sep 19. |