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Data sheet of TNF polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Pig |
| Host species | Rabbit |
| Applications | ELISA,IHC,WB-Re |
More info about TNF polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB16934 |
| Product name: | TNF polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against full length recombinant TNF. |
| Immunogen: | Recombinant protein corresponding to 154 amino acids of swine TNF. |
| Form: | Lyophilized |
| Recommend dilutions: | ELISA (1:2000-1:2500) Western Blot (1:1000) The optimal working dilution should be determined by the end user. |
| Storage buffer: | Lyophilized from 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide) |
| Storage instruction: | Store at 4°C on dry atmosphere. After reconstitution with 0.1 mL of deionized water, store at -20°C or lower. Aliquot to avoid repeated freezing and thawing. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Pig |
| Reactivity: | Pig |
| Application image: |  |
| Application image note: | Western blot using TNF polyclonal antibody (Cat # PAB16934) shows detection of recombinant Swine TNFa at about 16.9 KDa raised in yeast. The identity of the faint band at 135 KDa is not known. The protein was purified and resolved by SDS-PAGE, then transferred to PVDF membrane. Membrane was blocked with 3% BSA (BSA-30, diluted 1 : 10), and probed with 1 ug/mL primary antibody overnight at 4°C. After washing, membrane was probed with IRDye800™ Conjugated Goat Anti-Rabbit IgG for 45 min at room temperature. |
| Applications: | ELISA,IHC,WB-Re |
| Shipping condition: | Dry Ice |
| Publications: | Regulation of neutrophil apoptosis by tumor necrosis factor-alpha: requirement for TNFR55 and TNFR75 for induction of apoptosis in vitro.Murray J, Barbara JA, Dunkley SA, Lopez AF, Van Ostade X, Condliffe AM, Dransfield I, Haslett C, Chilvers ER. Blood. 1997 Oct 1;90(7):2772-83. |