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Data sheet of Ccl20 polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Mouse,Rat |
| Host species | Rabbit |
| Applications | ELISA,WB-Re |
More info about Ccl20 polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB16930 |
| Product name: | Ccl20 polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against full length recombinant Ccl20. |
| Gene id: | 20297 |
| Gene name: | Ccl20 |
| Gene alias: | CKb4|LARC|MIP-3A|MIP-3[a]|MIP3A|ST38|Scya20|exodus-1 |
| Gene description: | chemokine (C-C motif) ligand 20 |
| Immunogen: | Recombinant protein corresponding to full length mouse Ccl20. |
| Form: | Lyophilized |
| Recommend dilutions: | ELISA (1:10000) Western Blot (1:1000) The optimal working dilution should be determined by the end user. |
| Storage buffer: | Lyophilized from 20 mM KH2PO4, 150 mM NaCl, pH 7.2 |
| Storage instruction: | Store at 4°C on dry atmosphere. After reconstitution with 0.1 mL of deionized water, store at -20°C or lower. Aliquot to avoid repeated freezing and thawing. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Mouse |
| Reactivity: | Mouse,Rat |
| Application image: |  |
| Application image note: | Western blot using Ccl20 polyclonal antibody (Cat # PAB16930) shows detection of a band ~11 KDa in size corresponding to recombinant mouse Ccl20. Recombinant mouse Ccl20 was loaded on to an SDS-PAGE gel at 0.25 ug and after separation was transferred to nitrocellulose. The membrane was blocked with 1% BSA in TBST for 30 min at RT, followed by incubation with primary antibody diluted 1 : 1,000 in 1% BSA in TBST overnight at 4°C. After washes, the blot was reacted with secondary antibody HRP Goat anti-Rabbit IgG antibody diluted 1 : 40,000 in blocking buffer for 30 min at RT followed by reation with FemtoMax™ Chemiluminescent substrate. Data was collected using Bio-Rad VersaDoc® 4000 MP imaging system. |
| Applications: | ELISA,WB-Re |
| Shipping condition: | Dry Ice |
| Publications: | Th17 cytokines stimulate CCL20 expression in keratinocytes in vitro and in vivo: implications for psoriasis pathogenesis.Harper EG, Guo C, Rizzo H, Lillis JV, Kurtz SE, Skorcheva I, Purdy D, Fitch E, Iordanov M, Blauvelt A. J Invest Dermatol. 2009 Sep;129(9):2175-83. Epub 2009 Mar 19. |