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Data sheet of Il17f polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Mouse,Rat |
| Host species | Rabbit |
| Applications | ELISA,WB-Re |
More info about Il17f polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB16929 |
| Product name: | Il17f polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against full length recombinant Il17f. |
| Gene id: | 257630 |
| Gene name: | Il17f |
| Gene alias: | C87042|IL-17F |
| Gene description: | interleukin 17F |
| Immunogen: | Recombinant protein corresponding to full length mouse Il17f. |
| Form: | Lyophilized |
| Recommend dilutions: | ELISA (1:10000) Western Blot (1:1000) The optimal working dilution should be determined by the end user. |
| Storage buffer: | Lyophilized from 20 mM KH2PO4, 150 mM NaCl, pH 7.2 |
| Storage instruction: | Store at 4°C on dry atmosphere. After reconstitution with 0.1 mL of deionized water, store at -20°C or lower. Aliquot to avoid repeated freezing and thawing. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Mouse |
| Reactivity: | Mouse,Rat |
| Application image: |  |
| Application image note: | Western blot using Il17f polyclonal antibody (Cat # PAB16929) shows detection of a band ~18 KDa in size corresponding to recombinant mouse IL17f. Recombinant mouse IL17f was loaded on to an SDS-PAGE gel at 0.25 ug and after separation was transferred to nitrocellulose. The membrane was blocked with 1% BSA in TBST for 30 min at RT, followed by incubation with primary antibody diluted 1 : 1,000 in 1% BSA in TBST overnight at 4°. After washes, the blot was reacted with secondary antibody HRP Goat anti-Rabbit IgG antibody diluted 1 : 40,000 in blocking buffer for 30 min at RT followed by reation with FemtoMax™ Chemiluminescent substrate. Data was collected using Bio-Rad VersaDoc® 4000 MP imaging system. |
| Applications: | ELISA,WB-Re |
| Shipping condition: | Dry Ice |
| Publications: | Abundant c-Fas-associated death domain-like interleukin-1-converting enzyme inhibitory protein expression determines resistance of T helper 17 cells to activation-induced cell death.Yu Y, Iclozan C, Yamazaki T, Yang X, Anasetti C, Dong C, Yu XZ. Blood. 2009 Jul 30;114(5):1026-8. Epub 2009 May 8. |