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Data sheet of IL1B polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Dog,Human,Primates |
| Host species | Rabbit |
| Applications | ELISA,WB-Re |
More info about IL1B polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB16926 |
| Product name: | IL1B polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against full length recombinant IL1B. |
| Gene id: | 3553 |
| Gene name: | IL1B |
| Gene alias: | IL-1|IL1-BETA|IL1F2 |
| Gene description: | interleukin 1, beta |
| Immunogen: | Recombinant protein corresponding to human IL1B. |
| Form: | Lyophilized |
| Recommend dilutions: | ELISA (1:1000-1:5000) Western Blot (1:500-1:2000) The optimal working dilution should be determined by the end user. |
| Storage buffer: | Lyophilized from 20 mM KH2PO4, 150 mM NaCl, pH 7.2 |
| Storage instruction: | Store at 4°C on dry atmosphere. After reconstitution with 0.1 mL of deionized water, store at -20°C or lower. Aliquot to avoid repeated freezing and thawing. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Human |
| Reactivity: | Dog,Human,Primates |
| Application image: |  |
| Application image note: | Western blot showing detection of recombinant human IL1B by IL1B polyclonal antibody (Cat # PAB16926). Recombinant human IL1B was loaded on to an SDS-PAGE gel at 0.1 ug and after separation, transferred to nitrocellulose. The expected band is approximately 18 KDa in size. The membrane was blocked with 1% BSA in TBST for 30min at RT, followed by incubation with primary antibody diluted 1 : 1,000 in1% BSA in TBST overnight at 4°C. After washes, the blot was reacted with secondary antibody HRP Goat anti-Rabbit IgG antibody diluted 1 : 40,000 in blocking buffer for 30 min at RT. Data was collected using Bio-Rad Versa Doc® 4000 MP imaging system. |
| Applications: | ELISA,WB-Re |
| Shipping condition: | Dry Ice |
| Publications: | Molecular cloning of the interleukin-1 beta converting enzyme.Cerretti DP, Kozlosky CJ, Mosley B, Nelson N, Van Ness K, Greenstreet TA, March CJ, Kronheim SR, Druck T, Cannizzaro LA, et al.. Science. 1992 Apr 3;256(5053):97-100. |