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IL17F polyclonal antibody

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Data sheet of IL17F polyclonal antibody

BrandAbnova
Product typePrimary antibodies
ReactivityHuman
Host speciesRabbit
ApplicationsELISA,WB-Re

More info about IL17F polyclonal antibody

Brand: Abnova
Reference: PAB16921
Product name: IL17F polyclonal antibody
Product description: Rabbit polyclonal antibody raised against full length recombinant IL17F.
Gene id: 112744
Gene name: IL17F
Gene alias: IL-17F|ML-1|ML1
Gene description: interleukin 17F
Immunogen: Recombinant protein corresponding to full length human IL17F.
Form: Lyophilized
Recommend dilutions: ELISA (1:1000-1:5000)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.
Storage buffer: Lyophilized from 20 mM KH2PO4, 150 mM NaCl, pH 7.2
Storage instruction: Store at 4°C on dry atmosphere.
After reconstitution with 0.1 mL of deionized water, store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing.
Product type: Primary antibodies
Host species: Rabbit
Antigen species / target species: Human
Reactivity: Human
Application image: PAB16921-50-outsr-1.jpg
Application image note: Western blot using IL17F polyclonal antibody (Cat # PAB16921) shows detection of a band ~15 kDa in size corresponding to recombinant human IL17F. After transfer, the membrane was blocked overnight with 3% BSA in TBS followed by reaction with primary antibody at a 1 : 1,000 dilution. Detection occurred using DyLight 649 conjugated anti-Rabbit IgG secondary antibody diluted 1 : 20,000 in blocking buffer. Image was captured using VersaDoc MP 4000 imaging system (Bio-Rad).
Applications: ELISA,WB-Re
Shipping condition: Dry Ice
Publications: IL-17s adopt a cystine knot fold: structure and activity of a novel cytokine, IL-17F, and implications for receptor binding.Hymowitz SG, Filvaroff EH, Yin JP, Lee J, Cai L, Risser P, Maruoka M, Mao W, Foster J, Kelley RF, Pan G, Gurney AL, de Vos AM, Starovasnik MA.
EMBO J. 2001 Oct 1;20(19):5332-41.

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