Cart 0 Product Products (empty)
No products
To be determined Shipping
0,00 € Total
Prices are tax excluded
Product successfully added to your shopping cart
Quantity
Total
There are 0 items in your cart. There is 1 item in your cart.
Total products (tax excl.)
Total shipping (tax excl.) To be determined
Total (tax excl.)
Data sheet of VEGFA polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Bovine |
| Host species | Rabbit |
| Applications | ELISA,WB-Re |
More info about VEGFA polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB16916 |
| Product name: | VEGFA polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against full length recombinant VEGFA. |
| Gene id: | 281572 |
| Gene name: | VEGFA |
| Gene alias: | VEGF |
| Gene description: | vascular endothelial growth factor A |
| Immunogen: | Recombinant protein corresponding to 164 amino acids of bovine VEGFA. |
| Form: | Lyophilized |
| Recommend dilutions: | Western Blot (1:333) The optimal working dilution should be determined by the end user. |
| Storage buffer: | Lyophilized from 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide) |
| Storage instruction: | Store at 4°C on dry atmosphere. After reconstitution with 0.1 mL of deionized water, store at -20°C or lower. Aliquot to avoid repeated freezing and thawing. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Bovine |
| Reactivity: | Bovine |
| Application image: |  |
| Application image note: | Western blot using VEGFA polyclonal antibody (Cat # PAB16916) shows detection of recombinant bovine VEGFA at 17-19.2 KDa (arrowhead). Approximately 2 ug of recombinant protein was loaded per lane onto a 4-20% gradient gel followed by transfer to PVDF membrane. The membrane was blocked using 3% BSA diluted 1 : 10. The primary antibody was used at a 1 : 333 dilution and was incubated with the blot for 2h at room temperature. The membrane was washed and reacted with a 1 : 10,000 dilution of IRDyeâ„¢800 Conjugated Affinity Purified Goat-anti-Rabbit IgG [H&L] MX10. Molecular weight estimation was made by comparison to prestained MW markers. |
| Applications: | ELISA,WB-Re |
| Shipping condition: | Dry Ice |
| Publications: | Vascular endothelial growth factor: a new member of the platelet-derived growth factor gene family.Tischer E, Gospodarowicz D, Mitchell R, Silva M, Schilling J, Lau K, Crisp T, Fiddes JC, Abraham JA. Biochem Biophys Res Commun. 1989 Dec 29;165(3):1198-206. |