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Data sheet of RRM2B polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Human |
| Host species | Rabbit |
| Applications | WB,ELISA |
More info about RRM2B polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB16911 |
| Product name: | RRM2B polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against synthetic peptide of RRM2B . |
| Gene id: | 50484 |
| Gene name: | RRM2B |
| Gene alias: | DKFZp686M05248|MGC102856|MGC42116|p53R2 |
| Gene description: | ribonucleotide reductase M2 B (TP53 inducible) |
| Immunogen: | A synthetic peptide corresponding to N-terminus of human RRM2B . |
| Form: | Liquid |
| Recommend dilutions: | ELISA (1:3000) Western Blot (1 ug/mL) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide) |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Human |
| Reactivity: | Human |
| Application image: |  |
| Application image note: | Western blot using RRM2B polyclonal antibody (Cat # PAB16911) shows detection of recombinant (Lanes 1 and 3) and endogenous protein (Lanes 1 to 4) in whole cell extracts from transfected 293T. Lane 1 contains purified recombinant human p53R2. Lane 2 contains 293T cells transfected with control vector. Lane 3 contains 293T transfected with p53R2-myc. Lane 4 : 293T transfected with ScRNA. Lane 5 : 293T tranfected with p53R2 SiRNA The band at 40.7 KDa, indicated by the bottom arrowhead, corresponds to the expected molecular weight of endogenous RRM2B. The band with the middle arrow corresponds to myc-tagged p53R2 at 41.9 KDa. The highest band at 44.3 KDa corresponds to recombinant p53R2. Primary antibody was diluted to 1 ug/mL and incubated overnight at 4°C. ECL detection was used. |
| Applications: | WB,ELISA |
| Shipping condition: | Dry Ice |
| Publications: | PKB/Akt: a key mediator of cell proliferation, survival and insulin responses?Lawlor MA, Alessi DR. J Cell Sci. 2001 Aug;114(Pt 16):2903-10. |